Initiator Elements Function to Determine the Activity State of BX-C Enhancers

Article Article scientifique Article Initiator Elements Function to Determine the Activity State of BX-C Enhancers eng 2010 author Iampietro Carole 562568 author Gummalla Maheshwar Reddy 649421 author Mutero-Maeda Annick 134426 author Karch François 26338 author Maeda Robert 539408 e1001260 10.1371/journal.pgen.1001260 1553-7390 http://dx.plos.org/10.1371/journal.pgen.1001260 A >300 kb cis-regulatory region is required for the proper expression of the three bithorax complex (BX-C) homeotic genes. Based on genetic and transgenic analysis, a model has been proposed in which the numerous BX-C cis-regulatory elements are spatially restricted through the activation or repression of parasegment-specific chromatin domains. Particular early embryonic enhancers, called initiators, have been proposed to control this complex process. Here, in order to better understand the process of domain activation, we have undertaken a systematic in situ dissection of the iab-6 cis-regulatory domain using a new method, called InSIRT. Using this method, we create and genetically characterize mutations affecting iab-6 function, including mutations specifically modifying the iab-6 initiator. Through our mutagenesis of the iab-6 initiator, we provide strong evidence that initiators function not to directly control homeotic gene expression but rather as domain control centers to determine the activity state of the enhancers and silencers within a cis-regulatory domain. Dewey 590 44 Département de génétique et évolution 162 false PLOS genetics 6 12 4b8f8668-5357-4be2-bbdc-2e4451cafa8d Article (Published version) Public journal.pgen.1001260.pdf 816084 application/pdf openAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Initiator Elements Function to Determine the Activity State of BX-C Enhancers	PLOS genetics			2010	540	500

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Iampietro, Carole