Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish

Article Article scientifique Article Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish eng 2020 author Castanon Ortega Irinka 664714 author Hannich Joerg Thomas 693454 author Abrami Laurence 502416 author Huber F. author Dubois Marion 552271 author Muller Markus Johann 125264 author van der Goot F. G. author Gonzalez Gaitan Marcos 645929 10.1038/s41467-020-17196-3 2041-1723 http://www.nature.com/articles/s41467-020-17196-3 Oriented cell division is a fundamental mechanism to control asymmetric stem cell division, neural tube elongation and body axis extension, among other processes. During zebrafish gastrulation, when the body axis extends, dorsal epiblast cells display divisions that are robustly oriented along the animal-vegetal embryonic axis. Here, we use a combination of lipidomics, metabolic tracer analysis and quantitative image analysis to show that sphingolipids mediate spindle positioning during oriented division of epiblast cells. We identify the Wnt signaling as a regulator of sphingolipid synthesis that mediates the activity of serine palmitoyltransferase (SPT), the first and rate-limiting enzyme in sphingolipid production. Sphingolipids determine the palmitoylation state of the Anthrax receptor, which then positions the mitotic spindle of dividing epiblast cells. Our data show how Wnt signaling mediates sphingolipid-dependent oriented division and how sphingolipids determine Anthrax receptor palmitoylation, which ultimately controls the activation of Diaphanous to mediate spindle rotation and oriented mitosis Dewey 540 39 Département de biochimie 144 false Nature Communications 11 3317 d4b4787a-dd1c-4845-87e8-45c5432bd469 Article (Published version) Public wntcontrolled_MG_2020.pdf 3465790 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish	Nature Communications			2020	514	181

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Dubois, Marion