Production of Specific Monoclonal Antibodies to Salmonella typhi Flagellin and Possible Application to Immunodiagnosis of Typhoid Fever

Article Article scientifique Article Production of Specific Monoclonal Antibodies to Salmonella typhi Flagellin and Possible Application to Immunodiagnosis of Typhoid Fever eng 1990 author Sadallah Fatiha 23307 author Brighouse Guy 25562 author Del Giudice Giuseppe 22937 author Dayal Renu 54950 author Hocine Mouloud author Lambert Paul Henri 25121 59-64 10.1093/infdis/161.1.59 0022-1899 Four murine monoclonal antibodies (MAbs)to Salmonella typhi flagellin were produced. These MAbs did not react with eight other enterobacterial strains tested: Salmonella enteritidis, Salmonella typhimurium, Salmonella paratyphi A, Escherichia coli, Shigella flexneri, Shigella sonnei, Yersinia enterocolitica, and Campylobacter jejuni. Allfour MAbscross-reactedwith Salmonella muenchen flagellin indicating specificityfor d antigenic flagellar epitope. One MAb(C4) was selected to developa double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect S. typhi flagellin in serum samples. By use of this assay S. typhi flagellar antigen was detected in 95.5% of serum samples from patients with positive hemoculture for S. typhi, in 93.6% of samples from patients with positive serodiagnosis of typhoid fever, in 26% of samples collected from patients who were initially hemoculture-positive for S. typhi and who had undergone 7–8 d of chemotherapy, in 8.5% of samples from healthy persons from an endemic area, and in no samples from healthy persons from a nonendemic area. The presence of high levelsof flagellin antibody titers did not interfere with the antigen detection. The detection of S. typhi flagellar antigen in patient serum may have practical value for rapid diagnosis of typhoid fever. Dewey 616.07 137 Département de pathologie et immunologie 21F false The Journal of Infectious Diseases 161 1 76d9388d-0803-4be6-b6a3-899dd36a5af1 Article (Published version) Public Sadallah_JID_1990161059.pdf 4443440 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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Production of Specific Monoclonal Antibodies to Salmonella typhi Flagellin and Possible Application to Immunodiagnosis of Typhoid Fever	The Journal of Infectious Diseases			1990	472	445

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