Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins

Article Article scientifique Article Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins eng 2006 author Corcelle Véronique 546004 author Stieger B author Gjinovci Aslan 26086 author Wollheim Claes 25195 author Gauthier Benoît 129022 2826-2836 10.1016/j.yexcr.2006.05.007 16781709 0014-4827 Despite extensive studies, the hematopoietic versus hepatic origin of liver progenitor oval cells remains controversial. The aim of this study was to determine the origin of such cells after liver injury and to establish an oval cell line. Rat liver injury was induced by subcutaneous insertion of 2-AAF pellets for 7 days with subsequent injection of CCl(4). Livers were removed 9 to 13 days post-CCl(4) treatment. Immunohistochemistry was performed using anti-c-kit, OV6, Thy1, CK19, AFP, vWF and Rab3b. Isolated non-parenchymal cells were grown on mouse embryonic fibroblast, and their gene expression profile was characterized by RT-PCR. We identified a subpopulation of OV6/CK19/Rab3b-expressing cells that was activated in the periportal region of traumatized livers. We also characterized a second subpopulation that expressed the HSCs marker c-kit but not Thy1. Although we successfully isolated both cell types, OV6/CK19/Rab3b(+) cells fail to propagate while c-kit(+)-HSCs appeared to proliferate for up to 7 weeks. Cells formed clusters which expressed c-kit, Thy1 and albumin. Our results indicate that a bona fide oval progenitor cell population resides within the liver and is distinct from c-kit(+)-HSCs. Oval cells require the hepatic niche to proliferate, while cells mobilized from the circulation proliferate and transdifferentiate into hepatocytes without evidence of cell fusion. 2-Acetylaminofluorene/metabolism/pharmacology Animals Antigens, Differentiation/metabolism Antigens, Thy-1/immunology/metabolism Bone Marrow/metabolism Carbon Tetrachloride/pharmacology Cells, Cultured Female Hematopoietic Stem Cells/cytology/drug effects/metabolism Hepatocytes/cytology/drug effects/metabolism Immunohistochemistry Liver/cytology/drug effects/growth & development Liver Regeneration Male Mice Rats Rats, Sprague-Dawley Stem Cells/cytology/metabolism Dewey 612 134 Département de physiologie cellulaire et métabolisme 21C false Experimental cell research 312 15 209a454a-0ead-4bf0-a5e0-fdb764b0b414 Article (Published version) Public characterization of two distinct liver progenitor cell subpopulations Corcelle et al. Exp. cell research 2006.pdf 705842 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins	Experimental cell research			2006	666	620

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