Biochemical Membrane Lipidomics during Drosophila Development

Article Article scientifique Article Biochemical Membrane Lipidomics during Drosophila Development eng 2013 author Guan Xue Li author Cestra Gianluca author Shui Guanghou author Kuhrs Antje 649234 author Schittenhelm Ralf B. author Hafen Ernst 544125 author van der Goot F. Gisou author Robinett Carmen C. author Gatti Maurizio author Gonzalez Gaitan Marcos 645929 author Wenk Markus R. 98-111 10.1016/j.devcel.2012.11.012 1534-5807 https://linkinghub.elsevier.com/retrieve/pii/S1534580712005321 Lipids play critical roles in energy homeostasis, membrane structure, and signaling. Using liquid chromatography and mass spectrometry, we provide a comprehensive semiquantification of lipids during the life cycle of Drosophila melanogaster (230 glycerophospholipids, 210 sphingolipids, 6 sterols and sterol esters, and 60 glycerolipids) and obtain biological insights through this biochemical resource. First, we find a high and constant triacylglycerol-to-membrane lipid ratio during pupal stage, which is nonobvious in the absence of nutrient uptake and tissue remodeling. Second, sphingolipids undergo specific changes in headgroup (glycosylation) and tail configurations (unsaturation and hydroxylation on sphingoid base and fatty acyls, respectively), which correlate with gene expression of known (GlcT/CG6437; FA2H/ CG30502) and putative (Cyt-b5-r/CG13279) enzymes. Third, we identify a gender bias in phosphoethanolamine-ceramides as a lead for future investigation into sexual maturation. Finally, we partially characterize ghiberti, required for male meiotic cytokinesis, as a homolog of mammalian serine palmitoyltransferase. Dewey 540 39 Département de biochimie 144 false Developmental Cell 24 1 acc6c47f-9635-4c2b-a79f-3fdf9ec7aa55 Article (Published version) Public Biochemical_MG_13.pdf 1504961 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Biochemical Membrane Lipidomics during Drosophila Development	Developmental Cell			2013	261	264

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Hafen, Ernst