The role of the DIF motif of the DnaJ (Hsp40) co-chaperone in the regulation of the DnaK (Hsp70) chaperone cycle

Article Article scientifique Article The role of the DIF motif of the DnaJ (Hsp40) co-chaperone in the regulation of the DnaK (Hsp70) chaperone cycle eng 2006 author Cogelja Cajo Gordana 542785 author Horne B. E. author Kelley William 28396 author Schwager Françoise 27468 author Georgopoulos Costa Panos 27799 author Genevaux Pierre 514343 12436-12444 10.1074/jbc.M511192200 16533811 0021-9258 http://www.jbc.org/content/281/18/12436.full.pdf To perform effectively as a molecular chaperone, DnaK (Hsp70) necessitates the assistance of its DnaJ (Hsp40) co-chaperone partner, which efficiently stimulates its intrinsically weak ATPase activity and facilitates its interaction with polypeptide substrates. In this study, we address the function of the conserved glycine- and phenylalanine-rich (G/F-rich) region of the Escherichia coli DnaJ in the DnaK chaperone cycle. We show that the G/F-rich region is critical for DnaJ co-chaperone functions in vivo and that despite a significant degree of sequence conservation among the G/F-rich regions of Hsp40 homologs from bacteria, yeast, or humans, functional complementation in the context of the E. coli DnaJ is limited. Furthermore, we found that the deletion of the whole G/F-rich region is mirrored by mutations in the conserved Asp-Ile/Val-Phe (DIF) motif contained in this region. Further genetic and biochemical analyses revealed that this amino acid triplet plays a critical role in regulation of the DnaK chaperone cycle, possibly by modulating a crucial step subsequent to DnaK-mediated ATP hydrolysis. Adenosine Triphosphate/chemistry Amino Acid Motifs Amino Acid Sequence Animals Conserved Sequence Escherichia coli/metabolism Escherichia coli Proteins/ chemistry/metabolism Glycine/chemistry HSP40 Heat-Shock Proteins/ chemistry/metabolism HSP70 Heat-Shock Proteins/ chemistry/metabolism Humans Hydrolysis Molecular Chaperones/chemistry Molecular Sequence Data Phenylalanine/chemistry Dewey 616 56 Département de médecine 221 false The Journal of biological chemistry 281 18 bd56fa32-53ec-4a9f-b69c-9fc85e6ada57 Article Restricted file1.pdf 490291 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de médecine

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The role of the DIF motif of the DnaJ (Hsp40) co-chaperone in the regulation of the DnaK (Hsp70) chaperone cycle	The Journal of biological chemistry			2006	549	0

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Cogelja Cajo, Gordana