Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae

Article Article scientifique Article Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae eng 2009 author Camblong Jurgi 134974 author Beyrouthy Nissrine 662490 author Guffanti Elisa 683154 author Schlaepfer Guillaume 538841 author Steinmetz Lars M author Stutz Françoise 64881 1534-1545 10.1101/gad.522509 19571181 1549-5477 Homology-dependent gene silencing, a phenomenon described as cosuppression in plants, depends on siRNAs. We provide evidence that in Saccharomyces cerevisiae, which is missing the RNAi machinery, protein coding gene cosuppression exists. Indeed, introduction of an additional copy of PHO84 on a plasmid or within the genome results in the cosilencing of both the transgene and the endogenous gene. This repression is transcriptional and position-independent and requires trans-acting antisense RNAs. Antisense RNAs induce transcriptional gene silencing both in cis and in trans, and the two pathways differ by the implication of the Hda1/2/3 complex. We also show that trans-silencing is influenced by the Set1 histone methyltransferase, which promotes antisense RNA production. Finally we show that although antisense-mediated cis-silencing occurs in other genes, trans-silencing so far depends on features specific to PHO84. All together our data highlight the importance of noncoding RNAs in mediating RNAi-independent transcriptional gene silencing. DNA-Binding Proteins/metabolism Gene Dosage/genetics Gene Expression Regulation, Fungal Gene Silencing Histone Deacetylases/metabolism Histone-Lysine N-Methyltransferase Proton-Phosphate Symporters/genetics RNA, Antisense/metabolism Saccharomyces cerevisiae/genetics/metabolism Saccharomyces cerevisiae Proteins/genetics/metabolism Suppression, Genetic/genetics Trans-Activators/metabolism Transcription Factors/metabolism Dewey 570 46 Département de biologie cellulaire 165 false Genes & development 23 13 95671a0e-a626-4f53-8f6f-752c6ca7427c Article (Published version) Restricted Camblong et al., 2009.pdf 937783 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae	Genes & development			2009	582	0

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Schlaepfer, Guillaume