Transient deregulation of canonical Wnt signaling in developing pyramidal neurons leads to dendritic defects and impaired behavior

Article Article scientifique Article Transient deregulation of canonical Wnt signaling in developing pyramidal neurons leads to dendritic defects and impaired behavior eng 2019 author Viale Beatrice 781695 author Song Lin author Petrenko Volodymyr 712248 author Wenger Combremont Anne-Laure 537885 author Contestabile Alessandro 0000-0001-6528-1403 838891 author Bocchi Riccardo 634138 author Salmon Patrick 505385 author Carleton Alan 676577 author An Lijia author Vutskits Laszlo 109383 author Kiss Jozsef Zoltan 22758 1487-1502.e6 10.1016/j.celrep.2019.04.026 31042475 2211-1247 https://www.sciencedirect.com/science/article/pii/S2211124719304917 During development, the precise implementation of molecular programs is a key determinant of proper dendritic development. Here, we demonstrate that canonical Wnt signaling is active in dendritic bundle-forming layer II pyramidal neurons of the rat retrosplenial cortex during dendritic branching and spine formation. Transient downregulation of canonical Wnt transcriptional activity during the early postnatal period irreversibly reduces dendritic arbor architecture, leading to long-lasting deficits in spatial exploration and/or navigation and spatial memory in the adult. During the late phase of dendritogenesis, canonical Wnt-dependent transcription regulates spine formation and maturation. We identify neurotrophin-3 as canonical Wnt target gene in regulating dendritogenesis. Our findings demonstrate how temporary imbalance in canonical Wnt signaling during specific time windows can result in irreversible dendritic defects, leading to abnormal behavior in the adult. Dewey 616.8 Dewey 617 Swiss National Science Foundation Wnt signaling regulates neuronal migration and differentiation in the cerebral cortex 31003A_159795 CH Project funding 53 Département de neurosciences fondamentales 21A 63 Département d'anesthésiologie, pharmacologie, soins intensifs et urgences 229 false 543 Anesthésie et neurosciences 883 536 Groupe Carleton Alan (neurosciences fondamentales) 876 Cell Reports 27 5 e12e9149-28dd-451d-bc0f-5676265f4a78 Article (Published version) Public 1-s2.0-S2211124719304917-main.pdf 6544115 application/pdf CC BY-NC-ND 3a7ca5d8-0aec-4c76-a7d5-0c33928358d3 Supplemental data Public 1-s2.0-S2211124719304917-mmc1.pdf 3870529 application/pdf CC BY-NC-ND Vignette Public 1-s2.0-S2211124719304917-fx1.jpg 70613 image/jpeg openAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales Faculté de médecine / Section de médecine clinique / Département d'anesthésiologie, pharmacologie, soins intensifs et urgences

Title	Published in	Year	Views	Downloads

Transient deregulation of canonical Wnt signaling in developing pyramidal neurons leads to dendritic defects and impaired behavior	Cell Reports	2019	370	181
Study of layer 6b cortical neurons in vitro		2016	668	4

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Wenger Combremont, Anne-Laure