Genetic polymorphism in Caulerpa taxifolia (Ulvophyceae) chloroplast DNA revealed by a PCR-based assay of the invasive Mediterranean strain

Article Article scientifique Article Genetic polymorphism in Caulerpa taxifolia (Ulvophyceae) chloroplast DNA revealed by a PCR-based assay of the invasive Mediterranean strain eng 2002-07-01 2002-07-01 author Fama Patrizia 532768 author Jousson Olivier 55774 author Zaninetti Louisette 24943 Administration de la faculté des sciences (SIAD) author Meinesz A. author Dini F. author Di Giuseppe G. author Millar A. J. K. author Pawlowski Jan Wojciech 23432 618-624 10.1046/j.1420-9101.2002.00418.x 1010-061X An invasive, cold-tolerant strain of the tropical green alga Caulerpa taxifolia was introduced recently in the Mediterranean Sea and along the Californian coast. We screened 50 aquarium and open-sea C. taxifolia specimens for the presence/absence of an intron located in the rbcL gene of chloroplast DNA. We also reanalysed a total of 229 sequences of the Internal Transcribed Spacer (ITS) of ribosomal DNA, combining previously published sequences from different studies with 68 new sequences to complement rbcL data. The introduced Mediterranean strain was found to be characterized by the absence of the rbcL intron and by the occurrence of a particular monomorphic ITS type. A PCR assay based on rbcL gene was developed to detect new introductions of the invasive strain of C. taxifolia. This rapid and inexpensive test could be useful to assist environment managers in the preservation of coastal marine ecosystems. Caulerpa taxifolia Chloroplast DNA Invasive species PCR assay rbcL intron Dewey 590 44 Département de génétique et évolution 162 false Journal of evolutionary biology 15 4 37d91e9f-81be-40b6-a511-cfbe0ac3ef42 Article (Published version) Restricted Fama_JEB_200215618.pdf 994805 application/pdf 6e14afce89a3edb0fd87d763002165166e835a573b18114046ab2e210c86283f restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Genetic polymorphism in Caulerpa taxifolia (Ulvophyceae) chloroplast DNA revealed by a PCR-based assay of the invasive Mediterranean strain	Journal of evolutionary biology			2002	60	0

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