Specific roles for the Ccr4-Not complex subunits in expression of the genome

Article Article scientifique Article Specific roles for the Ccr4-Not complex subunits in expression of the genome eng 2009 author Azzouz Nowel 644417 author Panasenko Olesya 629191 author Deluen Sagne Cécile 527476 author Hsieh Julien 631847 author Theiler Gregory 103743 author Collart Martine 0000-0001-6955-1773 27009 377-383 10.1261/rna.1348209 19155328 1355-8382 PMC2657018 https://rnajournal.cshlp.org/content/15/3/377.long In this work we used micro-array experiments to determine the role of each nonessential subunit of the conserved Ccr4-Not complex in the control of gene expression in the yeast Saccharomyces cerevisiae. The study was performed with cells growing exponentially in high glucose and with cells grown to glucose depletion. Specific patterns of gene deregulation were observed upon deletion of any given subunit, revealing the specificity of each subunit's function. Consistently, the purification of the Ccr4-Not complex through Caf40p by tandem affinity purification from wild-type cells or cells lacking individual subunits of the Ccr4-Not complex revealed that each subunit had a particular impact on complex integrity. Furthermore, the micro-arrays revealed that the role of each subunit was specific to the growth conditions. From the study of only two different growth conditions, revealing an impact of the Ccr4-Not complex on more than 85% of all studied genes, we can infer that the Ccr4-Not complex is important for expression of most of the yeast genome. Gene Expression Regulation, Fungal Genome, Fungal Multiprotein Complexes/isolation & purification/metabolism Protein Array Analysis Ribonucleases/isolation & purification/metabolism Saccharomyces cerevisiae/genetics/metabolism Saccharomyces cerevisiae Proteins/isolation & purification/metabolism Transcription Factors/isolation & purification/metabolism Dewey 616 54 Département de microbiologie et médecine moléculaire 21B false 58 Caractérisation génétique et biochimique du complexe Ccr4-Not conservé. 81 RNA 15 3 1289c1ac-aebe-4787-abac-81c31de3f040 Article (Published version) Restricted file1.pdf 1408935 application/pdf 63327143-dd97-4f93-8583-68ec2917579f Corrigendum Restricted RNA-2025-Azzouz-139-40.pdf 1336771 application/pdf 47b7e4c0ea11f9ed90a2d4f3f09dc519c0b9abec68b3c45ef18a9f73d5ef3da6 The authors have been made aware that the juxtaposition of lanes in Figure 2 that were not juxtaposed in the original blots is difficult to interpret critically without providing the original blots. The revised Figure 2 has been consolidated [...] and the original blots have now been provided as raw data files (Supplemental Fig. 4, below). The revised legend is also provided. The conclusions drawn from the data have not changed, and the authors apologize for any confusion this may have caused. 10.1261/rna.080301.124 39681413 restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Specific roles for the Ccr4-Not complex subunits in expression of the genome	RNA			2009	644	2

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