Tools for Regulated Gene Expression in the Chloroplast of Chlamydomonas

Livre Chapitre de livre Tools for Regulated Gene Expression in the Chloroplast of Chlamydomonas eng 2014-02-03 2014-02-03 author Rochaix Jean-David 25289 Administration de la faculté des sciences (SIAD) author Surzycki Raymond 527450 author Ramundo Silvia 648848 413-424 10.1007/978-1-62703-995-6_28 24599871 9781627039949 https://link.springer.com/10.1007/978-1-62703-995-6_28 The green unicellular alga Chlamydomonas reinhardtii has emerged as a very attractive model system for chloroplast genetic engineering. Algae can be transformed readily at the chloroplast level through bombardment of cells with a gene gun, and transformants can be selected using antibiotic resistance or phototrophic growth. An inducible chloroplast gene expression system could be very useful for several reasons. First, it could be used to elucidate the function of essential chloroplast genes required for cell growth and survival. Second, it could be very helpful for expressing proteins which are toxic to the algal cells. Third, it would allow for the reversible depletion of photosynthetic complexes thus making it possible to study their biogenesis in a controlled fashion. Fourth, it opens promising possibilities for hydrogen production in Chlamydomonas. Here we describe an inducible/repressible chloroplast gene expression system in Chlamydomonas in which the copper-regulated Cyc6 promoter drives the expression of the nuclear Nac2 gene encoding a protein which is targeted to the chloroplast where it acts specifically on the chloroplast psbD 5′-untranslated region and is required for the stable accumulation of the psbD mRNA and photosystem II. The system can be used for any chloroplast gene or transgene by placing it under the control of the psbD 5′-untranslated region. Chlamydomonas Chloroplast gene Chloroplast genetics Chloroplast transformation Inducible chloroplast gene expression Inducible promoter Trans-acting factor Dewey 580 Humana Press Totowa, NJ Methods in molecular biology 1132 45 Département des sciences végétales 163 false Chloroplast Biotechnology: Methods and Protocols Maliga, Pal 272544cb-9fae-4a45-8675-4cb4c81e50fa Book chapter (Published version) Restricted Article_scientifique_from_crossref-.pdf 351064 application/pdf cb6c31826b1686bc12fe9a170ae88cecd3c57522abe6456ce9d8b8e951e7dc8b restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Tools for Regulated Gene Expression in the Chloroplast of Chlamydomonas	Chloroplast Biotechnology: Methods and Protocols			2014	71	0

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Surzycki, Raymond