GRP78 as a marker of pre-eclampsia: an exploratory study

Article Article scientifique Article GRP78 as a marker of pre-eclampsia: an exploratory study eng 2009 author Laverriere A. author Landau R. author Charvet Igor 518424 author Irion Olivier 125635 author Bischof Paul 26504 author Morales M. author Cohen Marie-Benoîte 0000-0003-4621-5464 135615 569-574 10.1093/molehr/gap037 19482905 1360-9947 http://molehr.oxfordjournals.org/content/15/9/569.full.pdf Although the exact mechanisms that lead to shallow invasion or defective trophoblastic differentiation in pre-eclampsia are still unknown, it is widely admitted that the etiology of pre-eclampsia is a defect in trophoblast invasion of the uterine spiral arteries. We have previously observed that the status of a chaperone protein, glucose regulated protein 78 (GRP78) is associated with the invasive properties of cytotrophoblastic cells; we therefore hypothesized that circulating GRP78 could serve as a diagnostic tool in pre-eclampsia. In a prospective case-control study, we quantified GRP78 autoantibodies, complexes of GRP78 with autoantibodies and GRP78 (C-term fragment, N-term fragment and full-length GRP78) by ELISA. Plasma from women diagnosed with pre-eclampsia (n = 16), from women during the first trimester of pregnancy who subsequently developed pre-eclampsia (n = 10) and from healthy pregnant women (controls, n = 58 at term, n = 26 at first trimester) were analysed and compared. We observed no significant difference between pre-eclamptic and healthy pregnant women for autoantibodies-GRP78 complexes or total GRP78 at both first trimester and at delivery. In contrast, the ratio of C-terminal GRP78 over full length GRP78 was significantly different in plasma of pre-eclamptic patients as compared with controls both during first trimester (P < 0.004) and at term (P < 0.0001). Our findings suggest that circulating C-terminal GRP78 reflect the invasive properties of cells, and could be used as a predictive marker for pre-eclampsia early in pregnancy. Autoantibodies/immunology Biological Markers/analysis/*metabolism Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Gestational Age Heat-Shock Proteins/analysis/immunology/*metabolism Humans Immunohistochemistry Pre-Eclampsia/*diagnosis/*metabolism Pregnancy Pregnancy Trimester, First Dewey 617 Dewey 618 58 Département de pédiatrie, gynécologie et obstétrique 223 63 Département d'anesthésiologie, pharmacologie, soins intensifs et urgences 229 false 48 Groupe Clergue François (anesthésiologie) 71 584 Laboratoire de biologie des tumeurs gynécologiques et du développement 927 213 Martinez De Tejada Begona 272 Molecular human reproduction 15 9 7c87784b-43af-489f-8c7b-395939bf14b1 Article Restricted file1.pdf 200824 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique Faculté de médecine / Section de médecine clinique / Département d'anesthésiologie, pharmacologie, soins intensifs et urgences

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GRP78 as a marker of pre-eclampsia: an exploratory study	Molecular human reproduction			2009	643	2

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