Non-aqueous capillary electrophoresis with diode array and electrospray mass spectrometric detection for the analysis of selected steroidal alkaloids in plant extracts

Article Article scientifique Article Non-aqueous capillary electrophoresis with diode array and electrospray mass spectrometric detection for the analysis of selected steroidal alkaloids in plant extracts eng 2001 author Cherkaoui Samir 111258 author Bekkouche Khalid 517888 author Christen Philippe 23100 author Veuthey Jean-Luc 84889 321-328 10.1016/S0021-9673(01)00907-4 0021-9673 Nonaqueous capillary electrophoresis coupled to UV detection is described for the separation and determination of steroidal alkaloids. After optimization of electrophoretic parameters, including the electrolyte nature and the organic solvent composition, a reliable separation of solasodine and solanidine was achieved in a methanol-acetonitrile (20:80, v/v) mixture containing 25 mM ammonium acetate and 1 M acetic acid. For quantitative purpose, a fused-silica capillary with a bubble cell was used and detection was performed at low wavelength (195 nm). Method performances, including migration time and peak area reproducibility, linearity, sensitivity and accuracy, were also evaluated. The method was applied to determine solasodine in Solanum elaeagnifolium berries and Solanum sodomaeum leaves and seeds. To further improve sensitivity in the analysis of solasodine-related compounds, solanidine, demissidine and tomatidine, the developed method was interfaced with electrospray ionization mass spectrometry. In the case of solasodine, the detection limit was estimated at 3 μg/ml for NACE-UV and at 0.05 μg/ml for NACE-MS, in the selected ion-monituring mode. Non-aqueous capillary electrophoresis Plant materials Solanum spp Alkaloids Steroids Solasodine Solanidine Dewey 615 31 Section des sciences pharmaceutiques 17 false Journal of chromatography 922 1 2f2074fd-5bf8-4a61-bbdc-8631ec2d7709 Article (Published version) Restricted CHERKAOUI_JOC_2001328.pdf 186706 application/pdf 9db039f1507d36e03a86c7fbc8c99bb9ad744dab91df4b98825883c4c07cc8f1 restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Non-aqueous capillary electrophoresis with diode array and electrospray mass spectrometric detection for the analysis of selected steroidal alkaloids in plant extracts	Journal of chromatography			2001	56	0

Archive ouverte UNIGE

Bekkouche, Khalid