Analyse de la régulation du gène CBP lors des processus morphogénétiques de l'hydre

Diplôme Master Analyse de la régulation du gène CBP lors des processus morphogénétiques de l'hydre fre 2004 author Jean Philippe 516770 director Galliot Brigitte 0000-0001-7596-8284 29979 In Hydra, the transcription factor CREB is rapidly phosphorylated after amputation in the endodermal cells of the head-regenerating tips, a regulation that plays an important role in the head regeneration process. In bilaterians, the phosphorylated form of CREB binds to the CREB binding protein (CBP), which acts as a general co-activator of transcription. To find out if this regulatory cascade also occurs in Hydra, we analyzed the Hydra CBP protein which we found to be highly conserved during evolution. We detected a high level of CBP transcripts in the endodermal cells of the head-forming regions, either head-regeneration tips or buds growing from intact animals. We also noted hyper-acetylation of histone-4 in head-regenerating tips, and a marked negative effect of Scriptaid, a deacetylase inhibitory drug, on the efficacy of head regeneration. These preliminary results suggest that Hydra CBP could, via chromatin modifications, participate in the reactivation of the head re-development program. CAMP Response-Element Binding protein (CREB) CREB-binding protein (CBP) Hydra model system Evolution Regeneration Scriptaid Histone acetylation Dewey 590 Master de Biologie, option Génétique, Développement et Evolution Swiss National Science Foundation 3100-059462-99 CH 44 Département de génétique et évolution 162 false 960130d9-621f-4d89-b497-e16193df0b42 Master thesis Restricted 2004.06-JEAN_Philippe_diplome_final_2.pdf 6899628 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Analyse de la régulation du gène CBP lors des processus morphogénétiques de l'hydre				2004	92	0

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Jean, Philippe