Cell death pathways differ in several mouse models with motoneurone disease: analysis of pure motoneurone populations at a presymptomatic age

Article Article scientifique Article Cell death pathways differ in several mouse models with motoneurone disease: analysis of pure motoneurone populations at a presymptomatic age eng 2006-07-11 2006-09 author Perrin Florence E author Boisset Gaëlle 516469 author Lathuiliere Aurélien 0000-0002-5191-5214 557604 author Kato Ann Catherine 26384 1959-1972 10.1111/j.1471-4159.2006.04024.x 16831193 0022-3042 To identify candidate genes that are responsible for motoneurone degeneration, we combined laser capture microdissection with microarray technology. We analysed gene expression in pure motoneurones from two mouse mutants that develop motoneurone degeneration, progressive motor neuronopathy and wobbler. At a presymptomatic age, there was a significant differential expression of a restricted number of genes (25 and 72 in progressive motor neuronopathy and wobbler respectively, of 22 600 transcripts screened). We compared these results to our previous analyses in the copper-zinc superoxide dismutase mutant mouse (SOD1(G93A)) in which we observed a de-regulation of 27 genes. Some of these genes were de-regulated uniquely in one mouse mutant and some have already been identified in cell death pathways implicated in amyotrophic lateral sclerosis and animal models of motoneurone degeneration (i.e. de-regulation of intermediate filaments, axonal transport, the ubiquitin-proteasome system and excitotoxicity). One gene, vimentin, was differentially up-regulated in all mouse mutants; this main candidate gene has been confirmed by in situ hybridization and immunohistochemistry to be expressed in motoneurones in all mouse mutants. Furthermore, vimentin expression correlated with the state of motoneurone degeneration. These results identify early molecular changes that may be involved in the pathogenesis of motoneurones leading to cell death and favour a complex multipathway induction of the disease; surprisingly, there was no important modification in cell death-associated genes. This is the first study to show a clear difference in the genes that are de-regulated at an early stage in three different mouse models of motoneurone disease. Animals Animals, Newborn Cell Death Cell Separation / methods Computer Systems Cytoplasm / metabolism Disease Models, Animal Disease Progression Gene Expression Gene Expression Profiling Gene Expression Regulation Inclusion Bodies / pathology Lasers Mice Mice, Neurologic Mutants Microdissection Motor Neuron Disease / genetics Motor Neuron Disease / pathology Motor Neuron Disease / physiopathology Motor Neurons / metabolism Nerve Degeneration / genetics Nerve Degeneration / pathology Nerve Degeneration / physiopathology Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction RNA, Messenger / metabolism Spinal Cord / pathology Vimentin / genetics Vimentin / metabolism Dewey 616.8 53 Département de neurosciences fondamentales 21A false Journal of neurochemistry 98 6 972d5993-8836-405d-985c-75cc518cd13f Article (Published version) Restricted Perrin_et_al-2006-Journal_of_Neurochemistry.pdf 1161403 application/pdf CC BY restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales

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Cell death pathways differ in several mouse models with motoneurone disease: analysis of pure motoneurone populations at a presymptomatic age	Journal of neurochemistry			2006	176	1

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Boisset, Gaëlle