Microbial Communities of Conducting and Respiratory Zones of Lung-Transplanted Patients

Article Article scientifique Article Microbial Communities of Conducting and Respiratory Zones of Lung-Transplanted Patients eng 2016 author Beaume Marie-Emilie 684753 author Lazarevic Vladimir 0000-0002-6839-2536 684594 author Kohler Thilo 0000-0003-4889-9492 23327 author Gaia Nadia 800637 author Manuel Oriol author Aubert John-David author Baerlocher Loic Benoît 516315 author Farinelli Laurent Luca Denis 23905 author Gasche-Soccal Paola Marina Alessandra 33623 author Schrenzel Jacques 29184 author Van Delden Christian 24088 1749 10.3389/fmicb.2016.01749 27872615 1664-302X http://journal.frontiersin.org/article/10.3389/fmicb.2016.01749/full Background: Lung transplantation (LT) is a recognized treatment for end-stage pulmonary disease. Bacteria from the recipient nasopharynx seed the new lungs leading to infections and allograft damage. Understanding the characteristics and topological variations of the microbiota may be important to apprehend the pathophysiology of allograft dysfunction. Objectives: To examine the characteristics and relationship of bacterial compositions between conducting and respiratory zones of the allograft. Methods: We performed 16S rRNA gene sequencing on bronchial aspirates (BAs) and bronchoalveolar lavages (BALs) collected in pairs in 19 patients at several time-points post-LT. Results: The respiratory zone was characterized independently of the time post-LT by a higher bacterial richness than the conducting zone (p = 0.041). The phyla Firmicutes and Proteobacteria dominated both sampling zones, with an inverse correlation between these two phyla (Spearman r = -0.830). Samples of the same pair, as well as pairs from the same individual clustered together (Pseudo-F = 3.8652, p < 0.01). Microbiota of BA and BAL were more closely related in samples from the same patient than each sample type across different patients, with variation in community structure being mainly inter-individual (p < 0.01). Both number of antibiotics administered (p < 0.01) and time interval post-LT (p < 0.01) contributed to the variation in global microbiota structure. Longitudinal analysis of BA-BAL pairs of two patients showed dynamic wave like fluctuations of the microbiota. Conclusions: Our results show that post-transplant respiratory zones harbor higher bacterial richness, but overall similar bacterial profiles as compared to conductive zones. They further support an individual microbial signature following LT. conducting airways lung allograft lung transplantation microbiota respiratory airways Dewey 616 Dewey 617 54 Département de microbiologie et médecine moléculaire 21B 56 Département de médecine 221 57 Département de chirurgie 222 false 235 Perception du Quorum et gènes de virulence 301 562 Transplantation et hépatologie 905 Frontiers in microbiology 7 060913bd-5cf4-4961-b8ef-45e360a7bf61 Article (Published version) Public fmicb-07-01749.pdf 3100286 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine clinique / Département de chirurgie

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Microbial Communities of Conducting and Respiratory Zones of Lung-Transplanted Patients	Frontiers in microbiology			2016	548	465

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Baerlocher, Loic Benoît