Spontaneous cdc42 polarization independent of gdi-mediated extraction and actin-based trafficking

Article Article scientifique Article Spontaneous cdc42 polarization independent of gdi-mediated extraction and actin-based trafficking eng 2015-04-02 2015-04-02 author Bendezú Felipe O. author Vincenzetti Vincent author Vavylonis Dimitrios author Wyss Romain author Vogel Horst 515020 author Martin Sophie 0000-0002-5317-2557 403314 Département de biologie moléculaire et cellulaire (MOCEL) 1-30 e1002097 10.1371/journal.pbio.1002097 25837586 1544-9173 PMC4383620 https://dx.plos.org/10.1371/journal.pbio.1002097 <p>The small Rho-family GTPase Cdc42 is critical for cell polarization and polarizes spontaneously in absence of upstream spatial cues. Spontaneous polarization is thought to require dynamic Cdc42 recycling through Guanine nucleotide Dissociation Inhibitor (GDI)-mediated membrane extraction and vesicle trafficking. Here, we describe a functional fluorescent Cdc42 allele in fission yeast, which demonstrates Cdc42 dynamics and polarization independent of these pathways. Furthermore, an engineered Cdc42 allele targeted to the membrane independently of these recycling pathways by an amphipathic helix is viable and polarizes spontaneously to multiple sites in fission and budding yeasts. We show that Cdc42 is highly mobile at the membrane and accumulates at sites of activity, where it displays slower mobility. By contrast, a near-immobile transmembrane domain-containing Cdc42 allele supports viability and polarized activity, but does not accumulate at sites of activity. We propose that Cdc42 activation, enhanced by positive feedback, leads to its local accumulation by capture of fast-diffusing inactive molecules.</p> NIGMS NIH HHS R01GM098430 Swiss National Science Foundation Structure and function of ionotropic serotonin and acetylcholine receptors 133141 CH Projects European Commission Geometric control of the cell cycle in the fission yeast 260493 GEOMETRYCELLCYCLE EU FP7 true PLoS biology 13 4 78989e43-27f5-49f3-be1b-d89efa9c56e3 Article (Published version) Public Article-scientifique-from-unpaywall.pdf 9302341 application/pdf CC BY-4.0 cfbe36275d4d49417564d9968fd37f20d8818b530ee63e334c191fd81a29be55 openAccess

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Spontaneous cdc42 polarization independent of gdi-mediated extraction and actin-based trafficking	PLoS biology			2015	89	106

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Vogel, Horst