Expression of cell cycle proteins in T1a and T1b urothelial bladder carcinoma and their value in predicting tumor progression

Article Article scientifique Article Expression of cell cycle proteins in T1a and T1b urothelial bladder carcinoma and their value in predicting tumor progression eng 2004 author Mhawech P. author Greloz V. author Oppikofer Chantal 513820 author Szalay-Quinodoz I. author Herrmann François 70980 2367-2375 10.1002/cncr.20306 15160340 0008-543X BACKGROUND: Cell cycle proteins are important markers in predicting tumor behavior in urothelial carcinoma of the bladder. The objectives of this study were 1) to determine the expression levels of some of those markers in a series of patients with bladder carcinoma, 2) to define their value in distinguishing T1a (minimally invasive) from T1b (invasive) tumors, and 3) to evaluate their use as predictive factors in the progression of T1a and T1b tumors. METHODS: Tumor specimens from 101 patients were included (22 Ta specimens, 34 T1a specimens, 15 T1b specimens, and 30 T2 specimens). A tissue microarray from the 101 paraffin embedded tissue blocks was constructed. Immunohistochemistry for p16, p27, p21, p53, cyclin D1, and Ki-67 were performed. To evaluate T1a and T1b tumor progression, clinical and follow-up data were available for all 49 patients. RESULTS: Cyclin D1 and p27 were the only markers that showed a significant association with tumor stage and tumor grade (cyclin D1: P = 0.002 and P > 0.00, respectively; p27: P = 0.024 and P = 0.031, respectively). The results indicated that a combination of p21 (odds ratio, 5.7; 95% confidence interval [95% CI], 1.3-24.8 [P = 0.022]) and p16 (odds ratio, 3.7; 95% CI, 0.8-16.5 [P = 0.081]) may have potential use in distinguishing T1b tumors from T1a tumors. Finally, none of the markers examined were found to have predictive value for T1a and T1b tumor progression. CONCLUSIONS: The expression of cyclin D1 and p27 was associated with the most important prognostic factors (tumor stage and grade). The combination of p21 and p16 may have value in distinguishing T1b tumors from T1a tumors, although this finding must be evaluated in much larger series. Finally, none of the markers studied appeared to have predictive value for disease progression in patients with T1a and T1b urothelial bladder tumors. Aged Aged, 80 and over Bladder Neoplasms/*chemistry/pathology Carcinoma, Transitional Cell/*chemistry/pathology *Cell Cycle Proteins/metabolism Cyclin D1 Cyclin-Dependent Kinase Inhibitor p16/metabolism Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Cyclins/metabolism Disease Progression Female Humans Ki-67 Antigen/metabolism Male Middle Aged Neoplasm Invasiveness/*pathology Predictive Value of Tests Prognosis Tumor Markers, Biological/*analysis Tumor Suppressor Protein p53/metabolism Tumor Suppressor Proteins/metabolism Urothelium/*chemistry Dewey 616 Dewey 618.97 56 Département de médecine 221 65 Département de réadaptation et gériatrie 22B false Cancer 100 11 d807c26a-3c96-4f3a-9c44-e8e2acf5f768 Article Restricted file1.pdf 207984 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine clinique / Département de réadaptation et gériatrie

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Expression of cell cycle proteins in T1a and T1b urothelial bladder carcinoma and their value in predicting tumor progression	Cancer			2004	599	0

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