Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p

Article Article scientifique Article Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p eng 1999 author Schmitt Christel 506592 author Von Kobbe Alonso Cayetano 503637 author Bachi Angela author Panté Nelly author Rodrigues João P. author Boscheron Cécile 513425 author Rigaut Guillaume author Wilm Matthias Sebastian 504839 author Séraphin Bertrand author Carmo-Fonseca Maria author Izaurralde Elisa 22473 4332-4347 10.1093/emboj/18.15.4332 0261-4189 http://emboj.embopress.org/cgi/doi/10.1093/emboj/18.15.4332 Dbp5 is a DEAD‐box protein essential for mRNA export from the nucleus in yeast. Here we report the isolation of a cDNA encoding human Dbp5 (hDbp5) which is 46% identical to yDbp5p. Like its yeast homologue, hDbp5 is localized within the cytoplasm and at the nuclear rim. By immunoelectron microscopy, the nuclear envelope‐bound fraction of Dbp5 has been localized to the cytoplasmic fibrils of the nuclear pore complex (NPC). Consistent with this localization, we show that both the human and yeast proteins directly interact with an N‐terminal region of the nucleoporins CAN/Nup159p. In a conditional yeast strain in which Nup159p is degraded when shifted to the nonpermissive temperature, yDbp5p dissociates from the NPC and localizes to the cytoplasm. Thus, Dbp5 is recruited to the NPC via a conserved interaction with CAN/Nup159p. To investigate its function, we generated defective hDbp5 mutants and analysed their effects in RNA export by microinjection in Xenopus oocytes. A mutant protein containing a Glu→Gln change in the conserved DEAD‐box inhibited the nuclear exit of mRNAs. Together, our data indicate that Dbp5 is a conserved RNA‐dependent ATPase which is recruited to the cytoplasmic fibrils of the NPC where it participates in the export of mRNAs out of the nucleus. DEAD-box protein Nuclear pore complex RNA helicase RNA nuclear export Dewey 570 43 Département de biologie moléculaire 161 false EMBO Journal 18 15 dc3bd01c-557c-4b58-988f-036f1315e6d1 Article (Published version) Restricted Schmitt_et_al-1999-The_EMBO_Journal.pdf 563702 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p	EMBO Journal			1999	302	0

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