Reduced ability of neonatal and early-life bone marrow stromal cells to support plasmablast survival

Article Article scientifique Article Reduced ability of neonatal and early-life bone marrow stromal cells to support plasmablast survival eng 2006 author Pihlgren Bosch Maria Astrid Louise 508328 author Friedli Mathieu 511580 author Tougne Chantal 23068 author Rochat Anne-Françoise 26202 author Lambert Paul Henri 25121 author Siegrist Claire-Anne 26816 165-172 10.4049/jimmunol.176.1.165 16365407 0022-1767 In human infants (<1 year), circulating IgG Abs elicited in response to most T-dependent Ags rapidly decline and return to baseline within a few months after immunization for yet-unknown reasons. In mice immunized between 1 and 4 wk of age, a limited establishment of the bone marrow (BM) pool of long-lived plasma cells is observed. In this study, we show that tetanus toxoid (TT)-specific plasmablasts generated in the spleen are efficiently attracted in vitro and in vivo toward early-life BM stromal cells, which express adult levels of CXCL12. Similarly, adoptively transferred TT plasmablasts efficiently reach the BM compartment of 2-wk-old and adult mice. In contrast, TT plasmablasts fail to persist in the early-life BM compartment, as indicated by the persistence of a significantly lower number of TT plasmablasts in the early-life compartment than in the adult BM compartment 48 h after transfer. This limited persistence is associated with an increased rate of in vivo apoptosis of TT-specific plasmablasts that have reached the early-life BM and with a significantly lower survival rate of TT-specific plasmablasts cocultured on early-life BM stromal cells compared with adult BM stromal cells. Thus, early-life BM stromal cells fail to provide the molecular signals that support plasmablast survival and differentiation into surviving plasma cells. Adoptive Transfer Age Factors Animals Animals, Newborn Apoptosis/immunology Bone Marrow Cells/immunology Cell Differentiation/immunology Chemokine CXCL12 Chemokines, CXC/biosynthesis Chemotaxis, Leukocyte/immunology Flow Cytometry Hematopoietic Stem Cells/cytology/immunology Immunohistochemistry Mice Plasma Cells/cytology/immunology Reverse Transcriptase Polymerase Chain Reaction Spleen/cytology/immunology Stromal Cells/immunology Tetanus Toxoid/immunology Dewey 616.07 Dewey 618 137 Département de pathologie et immunologie 21F 58 Département de pédiatrie, gynécologie et obstétrique 223 false 143 Centre de Vaccinologie et d'Immunologie néonatale 177 The Journal of immunology 176 1 51b086a0-da84-4985-b6e4-1a1114906ad2 Article (Published version) Public 165-172.pdf 608198 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

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Reduced ability of neonatal and early-life bone marrow stromal cells to support plasmablast survival	The Journal of immunology			2006	608	407

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Friedli, Mathieu