The Ccr4-not complex regulates Skn7 through Srb10 kinase

Article Article scientifique Article The Ccr4-not complex regulates Skn7 through Srb10 kinase eng 2007 author Lenssen Eve 95422 author Azzouz Nowel 644417 author Michel Agnès 511247 author Landrieux Emilie author Collart Martine 0000-0001-6955-1773 27009 2251-2259 10.1128/EC.00327-06 17965252 1535-9786 The Ccr4-Not complex is a multifunctional regulatory platform composed of nine subunits that controls diverse cellular events including mRNA degradation, protein ubiquitination, and transcription. In this study, we identified the yeast Saccharomyces cerevisiae osmotic and oxidative stress transcription factor Skn7 as a new target for regulation by the Ccr4-Not complex. Skn7 interacts with Not1 in a two-hybrid assay and coimmunoprecipitates with Not5 in a Not4-dependent manner. Skn7-dependent expression of OCH1 and Skn7 binding to the OCH1 promoter are increased in not4Delta or not5Delta mutants. Skn7 purified from wild-type cells but not from not4Delta cells is associated with the Srb10 kinase. This kinase plays a central role in the regulation of Skn7 by Not4, since increased OCH1 expression in not4Delta cells requires Srb10. These results reveal a critical role for the Ccr4-Not complex in the mechanism of activation of Skn7 that is dependent upon the Srb10 kinase. Cyclin-Dependent Kinase 8 Cyclin-Dependent Kinases/metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation, Fungal Mannosyltransferases/metabolism Membrane Glycoproteins/metabolism Models, Biological Mutation Osmosis Oxidative Stress Plasmids/metabolism Promoter Regions, Genetic RNA, Messenger/metabolism Ribonucleases/metabolism Saccharomyces cerevisiae/genetics/metabolism Saccharomyces cerevisiae Proteins/metabolism Transcription Factors/metabolism Transcriptional Activation Dewey 616 Swiss National Science Foundation 3100A0-100793 CH 54 Département de microbiologie et médecine moléculaire 21B false 58 Caractérisation génétique et biochimique du complexe Ccr4-Not conservé 81 Eukaryotic cell 6 12 5ba47328-309b-4f86-a23a-89b4b2c93573 Article (Published version) Restricted Lenssen-2007-The Ccr4-not complex.pdf 378911 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

Title	Published in	Year	Views	Downloads

The Ccr4-not complex regulates Skn7 through Srb10 kinase	Eukaryotic cell	2007	696	0
Spontaneous rDNA copy number variation modulates Sir2 levels and epigenetic gene silencing		2005	1,072	983

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