Nucleoside diphosphate kinases fuel dynamin superfamily proteins with GTP for membrane remodeling

Article Article scientifique Article Nucleoside diphosphate kinases fuel dynamin superfamily proteins with GTP for membrane remodeling eng 2014 author Boissan M. author Montagnac G. author Shen Q. author Griparic Lorena 508299 author Guitton J. author Romao M. author Sauvonnet N. author Lagache T. author Lascu I. author Raposo G. author Desbourdes C. author Schlattner Uwe 27234 author Lacombe M.-L. author Polo S. author van der Bliek A. M. author Roux Aurélien 0000-0002-6088-0711 716946 author Chavrier P. 1510-1515 10.1126/science.1253768 0036-8075 https://www.sciencemag.org/lookup/doi/10.1126/science.1253768 Dynamin superfamily molecular motors use guanosine triphosphate (GTP) as a source of energy for membrane-remodeling events. We found that knockdown of nucleoside diphosphate kinases (NDPKs) NM23-H1/H2, which produce GTP through adenosine triphosphate (ATP)–driven conversion of guanosine diphosphate (GDP), inhibited dynamin-mediated endocytosis. NM23-H1/H2 localized at clathrin-coated pits and interacted with the proline-rich domain of dynamin. In vitro, NM23-H1/H2 were recruited to dynamin-induced tubules, stimulated GTP-loading on dynamin, and triggered fission in the presence of ATP and GDP. NM23-H4, a mitochondria-specific NDPK, colocalized with mitochondrial dynamin-like OPA1 involved in mitochondria inner membrane fusion and increased GTP-loading on OPA1. Like OPA1 loss of function, silencing of NM23-H4 but not NM23-H1/H2 resulted in mitochondrial fragmentation, reflecting fusion defects. Thus, NDPKs interact with and provide GTP to dynamins, allowing these motor proteins to work with high thermodynamic efficiency. Dewey 540 39 Département de biochimie 144 false Science 344 6191 0e5b2d0d-e650-41ea-be45-52ffe82dfe3e Article (Published version) Restricted Nucleoide_AR_14.pdf 4460313 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Nucleoside diphosphate kinases fuel dynamin superfamily proteins with GTP for membrane remodeling	Science			2014	301	0

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