Transcriptional activation by bidirectional RNA polymerase II elongation over a silent promoter

Article Article scientifique Article Transcriptional activation by bidirectional RNA polymerase II elongation over a silent promoter eng 2005 author Leupin Olivier author Attanasio Catia 103069 author Marguerat Samuel 508055 author Tapernoux Myriam 134941 author Antonarakis Stylianos 22813 author Conrad Bernard 96442 956-960 10.1038/sj.embor.7400502 16113646 1469-221X http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1369183/pdf/6-7400502.pdf Transcriptional interference denotes negative cis effects between promoters. Here, we show that promoters can also interact positively. Bidirectional RNA polymerase II (Pol II) elongation over the silent human endogenous retrovirus (HERV)-K 18 promoter (representative of 2.5 x 10(3) similar promoters genomewide) activates transcription. In tandem constructs, an upstream promoter activates HERV-K 18 transcription. This is abolished by inversion of the upstream promoter, or by insertion of a poly(A) signal between the promoters; transcription is restored by poly(A) signal mutants. TATA-box mutants in the upstream promoter reduce HERV-K 18 transcription. Experiments with the same promoters in a convergent orientation produce similar effects. A small promoter deletion partially restores HERV-K 18 activity, consistent with activation resulting from repressor repulsion by the elongating Pol II. Transcriptional elongation over this class of intragenic promoters will generate co-regulated sense-antisense transcripts, or, alternatively initiating transcripts, thus expanding the diversity and complexity of the human transcriptome. Animals Cells, Cultured Enhancer Elements, Genetic Humans Mice Mutation Plasmids/genetics RNA Polymerase II/ genetics Retroelements/genetics TATA Box/genetics/ physiology Transcription, Genetic Transcriptional Activation Dewey 616 56 Département de médecine 221 false EMBO reports 6 10 4b689e5a-c4ca-4faa-a993-664204149812 Article Restricted file1.pdf 137231 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de médecine

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Transcriptional activation by bidirectional RNA polymerase II elongation over a silent promoter	EMBO reports			2005	589	0

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Marguerat, Samuel