Human chorionic gonadotropin stimulates matrix metalloproteinases-2 and -9 in cytotrophoblastic cells and decreases tissue inhibitor of metalloproteinases-1, -2, and -3 in decidualized endometrial stromal cells

Article Article scientifique Article Human chorionic gonadotropin stimulates matrix metalloproteinases-2 and -9 in cytotrophoblastic cells and decreases tissue inhibitor of metalloproteinases-1, -2, and -3 in decidualized endometrial stromal cells eng 2008 author Fluhr Herbert author Bischof Dorina 506654 author Krenzer Stefanie author Licht Peter author Bischof Paul 26504 author Zygmunt Marek 1390-1395 10.1016/j.fertnstert.2007.08.023 18291374 0015-0282 http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271326&_user=9565874&_pii=S0015028207031512&_check=y&_origin=article&_zone=toolbar&_coverDate=31-Oct-2008&view=c&originContentFamily=serial&wchp=dGLzVBA-zSkzk&md5=b3d015438259c9c6dbf1dbdd1fcbb886/1-s2.0-S0015028207031512-main.pdf OBJECTIVE: To investigate the influence of hCG on trophoblastic matrix metalloproteinases (MMPs) -2 and -9 as well as endometrial tissue inhibitor of metalloproteinases (TIMPs) -1, -2, and -3. DESIGN: In vitro experiment. SETTING: Research laboratory at a university medical center. PATIENT(S): Women undergoing legal abortions and premenopausal women undergoing hysterectomy for benign reasons. INTERVENTION(S): Human first trimester cytotrophoblasts and decidualized endometrial stromal cells were incubated with recombinant hCG. MAIN OUTCOME MEASURE(S): Trophoblastic MMP-2 and -9 were analyzed by enzyme-linked immunosorbent assay (ELISA) and zymography, and endometrial TIMP-1, -2, and -3 were measured by real time reverse transcriptase-polymerase chain reaction and ELISA. RESULT(S): HCG increases the secretion of MMP-2 and -9 in cytotrophoblasts dose dependently. This effect occurs after 4 hours of incubation and becomes less pronounced after 24 hours. In contrast, TIMP-1, -2, and -3 are significantly reduced by hCG in endometrial stromal cells in a time- and dose-dependent manner. CONCLUSION(S): These results suggest a regulatory role of hCG on the MMP/TIMP system at the implantation site. By increasing trophoblastic MMP secretion and reducing endometrial TIMP expression, hCG may be an important tool for the invading embryo to regulate the depth of its implantation. Cells, Cultured Chorionic Gonadotropin/genetics/*metabolism Decidua/*metabolism Endometrium/metabolism Female Gene Expression Humans Matrix Metalloproteinase 2/genetics/*metabolism Matrix Metalloproteinase 9/genetics/*metabolism Stromal Cells/metabolism Tissue Inhibitor of Metalloproteinases/*genetics/metabolism Trophoblasts/*metabolism Dewey 618 58 Département de pédiatrie, gynécologie et obstétrique 223 false Fertility and sterility 90 4 Suppl 7e18207a-da86-4576-a7ae-adf182006b8f Article Restricted file1.pdf 166232 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

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Human chorionic gonadotropin stimulates matrix metalloproteinases-2 and -9 in cytotrophoblastic cells and decreases tissue inhibitor of metalloproteinases-1, -2, and -3 in decidualized endometrial stromal cells	Fertility and sterility			2008	614	0

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