Phosphorylation of CREB Ser142 Regulates Light-Induced Phase Shifts of the Circadian Clock

Article Article scientifique Article Phosphorylation of CREB Ser142 Regulates Light-Induced Phase Shifts of the Circadian Clock eng 2002-04-11 author Gau Daniel author Lemberger Thomas author von Gall Charlotte author Kretz Oliver author Le Minh Nguyet 506579 author Gass Peter author Schmid Wolfgang author Schibler Ulrich 22687 Département de biologie moléculaire et cellulaire (MOCEL) author Korf Horst W. author Schütz Günther 245-253 10.1016/S0896-6273(02)00656-6 11970866 0896-6273 Biological rhythms are driven in mammals by a central circadian clock located in the suprachiasmatic nucleus (SCN). Light-induced phase shifting of this clock is correlated with phosphorylation of CREB at Ser133 in the SCN. Here, we characterize phosphorylation of CREB at Ser142 and describe its contribution to the entrainment of the clock. In the SCN, light and glutamate strongly induce CREB Ser142 phosphorylation. To determine the physiological relevance of phosphorylation at Ser142, we generated a mouse mutant, CREBS142A, lacking this phosphorylation site. Light-induced phase shifts of locomotion and expression of c-Fos and mPer1 in the SCN are significantly attenuated in CREBS142A mutants. Our findings provide genetic evidence that CREB Ser142 phosphorylation is involved in the entrainment of the mammalian clock and reveal a novel phosphorylation-dependent regulation of CREB activity. Dewey 570 43 Département de biologie moléculaire 161 false Neuron 34 2 57543cd9-f1aa-4873-9e12-9a1ab942f319 Article (Published version) Restricted Gau_NEUR_200234245.pdf 475889 application/pdf e613c1c300cacbabca429d5c25149490fdad1a5594cc7e7f0b88be7a1a173203 restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Phosphorylation of CREB Ser142 Regulates Light-Induced Phase Shifts of the Circadian Clock	Neuron			2002	84	0

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Le, Minh Nguyet