A nested deletion approach to generate Cre deleter mice with progressive Hox profiles

Article Article scientifique Article A nested deletion approach to generate Cre deleter mice with progressive Hox profiles eng 2002 author Herault Yann 90304 author Kmita-Cunisse Marie-Thérèse 117087 author Sawaya Chadi Charles 502643 author Duboule Denis 28643 185-191 11902682 0214-6282 In mice, the loxP/Cre recombinase-dependent system of recombination offers powerful possibilities for engineering genetic configurations of interest. This system can also be advantageously used for conditional mutagenesis in vivo, whenever such an approach is required due to deleterious effects of either one mutation, or a combination thereof. Here, we report on the production of an allelic series of insertions of a Hoxd11/Cre fusion transgene at different positions within the HoxD complex, in order to produce the CRE recombinase with a 'Hox profile' progressively more extended. We used the R26R (R26R) reporter mouse line to functionally assess the distribution and efficiency of the CRE enzyme and discuss the usefulness of these various lines as deleter strains. Cre deleter mice Hox D TAMERE Dewey 590 44 Département de génétique et évolution 162 false The International journal of developmental biology 46 1 9632adc7-8bc3-4c62-b242-af6109369db0 Article (Published version) Public Y.Hérault_6.pdf 189821 application/pdf openAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

Title	Published in	Access level	OA Policy	Year	Views	Downloads

A nested deletion approach to generate Cre deleter mice with progressive Hox profiles	The International journal of developmental biology			2002	467	123

Archive ouverte UNIGE

Sawaya, Chadi Charles