Potential of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of freshwater zooplankton: a pilot study with three Eudiaptomus (Copepoda: Diaptomidae) species

Article Article scientifique Article Potential of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of freshwater zooplankton: a pilot study with three Eudiaptomus (Copepoda: Diaptomidae) species eng 2012 author Riccardi Nicoletta author Lucini Lara Maria Stella 47259 author Benagli Cinzia author Welker Martin author Wicht Barbara author Tonolla Mauro Amedeo 45173 484-492 10.1093/plankt/fbs022 0142-7873 The accurate identification of individuals in zooplankton samples is a crucial step in many plankton studies. Up to now, this has been done primarily by microscopic analysis of morphological characters, and new molecular methodologies are still relatively rarely applied. Another promising technology is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which has had a major impact in applied and systematic microbiology, where it is used for routine high throughput identification of bacteria and fungi. For the present study, we developed a protocol for the rapid acquisition of mass spectra from whole individual copepods. The final protocol enabled us to obtain mass spectra with more than 100 distinct peaks in the mass range of 2000–20 000 Da. A comparison of the mass spectra of three species of Eudiaptomus showed that they could all be clearly discriminated, whereas the mass spectra of different developmental stages and sexes of each particular species were highly similar. Further, a discrimination of con-specific individuals from different habitats was achieved, at least partly, even without extensive optimization of the analytical and statistical procedures. These results indicate the feasibility of identifying copepods by a rapid and simple MALDI-TOF MS analysis, e.g. for population ecology studies. Dewey 580 Open Access - Licence nationale Oxford University Press 45 Département des sciences végétales 163 false Journal of Plankton Research 34 6 f31b0442-4742-4a0a-847a-8528a4b55b16 Article (Published version) Public fbs022.pdf 293600 application/pdf OA NATIONAL openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Potential of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of freshwater zooplankton: a pilot study with three Eudiaptomus (Copepoda: Diaptomidae) species	Journal of Plankton Research			2012	417	166

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Lucini, Lara Maria Stella