Characterization of reactions catalysed by yeast phosphatidylinositol synthase

Article Article scientifique Article Characterization of reactions catalysed by yeast phosphatidylinositol synthase eng 1993 author Klezovitch Olga 27830 author Brandenburger Yves 46977 author Geindre Michèle 25800 author Deshusses Jacques 24866 256-260 10.1016/0014-5793(93)80598-o 8385029 0014-5793 The nature of reactions catalysed by yeast phosphatidylinositol synthase expressed in E. coli has been investigated. The single enzyme is shown to carry both CDP-diacylglycerol-dependent incorporation of inositol into phosphatidylinositol (Km for inositol of 0.090 mM) and a CDP-diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol (Km for inositol of 0.066 mM). The exchange reaction and reversal of phosphatidylinositol synthase were both stimulated by CMP, but had different optimum pH and requirements for substrates. These results suggest that CMP-stimulated exchange and CMP-dependent reverse reactions are distinct processes catalysed by the same enzyme, phosphatidylinositol synthase. CDP-diacylglycerol Phosphatidylinositol synthase Exchange reaction Saccharomyces cerevisiae Dewey 540 28 Section de chimie et biochimie 14 false FEBS letters 320 3 bae107c6-f583-4772-8a4d-2d4e969881d7 Article (Published version) Restricted O.Klezovitch.pdf 471166 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie

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Characterization of reactions catalysed by yeast phosphatidylinositol synthase	FEBS letters			1993	662	1

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Brandenburger, Yves