Ablation of islet endocrine cells by targeted expression of hormone-promoter-driven toxigenes

Article Article scientifique Article Ablation of islet endocrine cells by targeted expression of hormone-promoter-driven toxigenes eng 1994 author Herrera Pedro Luis 0000-0003-0771-9504 23981 author Huarte Joachim 22247 author Zufferey Romain Nicolas 24114 author Nichols Anthony Charles 23439 author Mermillod Bernadette 22748 author Philippe Jacques 26481 author Muniesa Lorda Pedro 45204 author Sanvito Francesca Maria Angelica 26679 author Orci Lelio 25002 author Vassalli Jean-Dominique 21998 12999-13003 10.1073/pnas.91.26.12999 7809163 0027-8424 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC45568/pdf/pnas01477-0654.pdf Ontogenic relationships between the different types of endocrine cells in the islets of Langerhans were explored by generating transgenic mouse embryos in which cells transcribing the glucagon, insulin, or pancreatic polypeptide genes were destroyed through the promoter-targeted expression of the diphtheria toxin A chain. Embryos lacking glucagon- or insulin-containing cells did not exhibit alterations in the development of the nontargeted islet cell types, whereas embryos lacking pancreatic polypeptide gene-expressing cells also lacked pancreatic insulin- and somatostatin-containing cells. These results show that neither glucagon nor insulin gene-expressing cells are essential for the differentiation of the other islet endocrine-cell types. These results also suggest that pancreatic polypeptide gene-expressing cells are indispensable for the differentiation of islet beta and delta cells because the former produce a necessary paracrine or endocrine factor and/or operate through a cell-lineage relationship. Animals Cell Death Diphtheria Toxin/administration & dosage Glucagon/genetics Insulin/genetics Islets of Langerhans/ cytology/embryology Mice Mice, Transgenic Pancreatic Polypeptide/genetics Promoter Regions, Genetic Somatostatin/metabolism Dewey 616 Swiss National Science Foundation 31-34088.92 56 Département de médecine 221 false 272 Types cellulaires pancréatiques pendant l'ontogénèse 522 Proceedings of the National Academy of Sciences of the United States of America 91 26 16937983-d689-4085-b2a6-5ed5a27e1bcc Article (Published version) Restricted file1.pdf 1434256 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de médecine

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Ablation of islet endocrine cells by targeted expression of hormone-promoter-driven toxigenes	Proceedings of the National Academy of Sciences of the United States of America			1994	638	1

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Muniesa Lorda, Pedro