Computerized classification of two-dimensional gel electrophoretograms by correspondence analysis and ascendant hierarchical clustering

Article Article scientifique Article Computerized classification of two-dimensional gel electrophoretograms by correspondence analysis and ascendant hierarchical clustering eng 1988 author Pun Thierry 22776 author Hochstrasser Denis 26422 author Appel Ron David 22305 author Funk Matthieu 67298 author Augsburger Valérie Laurence 45100 author Pellegrini Christian 24713 3-9 3154957 0954-6642 http://www.ncbi.nlm.nih.gov/pubmed/3154957 A powerful data processing methodology for analysis and classification of two-dimensional gels is introduced. The approach is based on correspondence analysis (CA) and ascendant hierarchical classification (AHC), and significantly differs from the more classical principal-component decomposition. Starting with a series of gels, each having a large number of spots, CA allows their representation in a factorial space of reduced dimension; classification into meaningful groups is then performed using AHC. Simultaneous representation of both spots and gels in the same space can be done. This precisely indicates the key spots pertinent for the classification, and therefore the characteristic proteins representative of a particular class of gels (i.e. of a particular disease or biological status). In addition, knowledge of these characteristic spots greatly simplifies the screening of future gels. After a brief overview of the Mélanie system for analyzing 2D gels, the theory of correspondence analysis and ascendant hierarchical classification is summarized. Equations are given that are easily ammenable to computation. How classification of two-dimensional gel electrophoretograms is accomplished is then detailed. Experimental results support the power of this approach. Cluster Analysis Electrophoresis, Gel, Two-Dimensional/classification Electrophoresis, Gel, Two-Dimensional/methods Factor Analysis, Statistical Image Interpretation, Computer-Assisted/methods Proteins/analysis Dewey 025.063 13 Centre universitaire d'informatique 0D3 32 Département d'informatique 18 false 895 Computer Vision and Multimedia Laboratory 730 Geneva Artificial Intelligence Laboratory 725 Multimodal Interaction Group Applied and theoretical electrophoresis 1 1 63cf9cae-e716-4786-ad97-8e3378bd1345 Article (Published version) Public 1988Pun_Comp_classif_2Dgel.pdf 1429170 application/pdf openAccess Centres et instituts / Centre universitaire d'informatique Faculté des sciences / Département d'informatique

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Computerized classification of two-dimensional gel electrophoretograms by correspondence analysis and ascendant hierarchical clustering	Applied and theoretical electrophoresis			1988	523	906

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Augsburger, Valérie Laurence