Nontemplated bases at the 5' ends of Tacaribe virus mRNAs

Article Article scientifique Article Nontemplated bases at the 5' ends of Tacaribe virus mRNAs eng 1990 author Raju Ramasamy 35807 author Raju Latha author Hacker David Lynn 42012 author Garcin Dominique 24296 author Compans Richard author Kolakofsky Daniel 25420 53-59 10.1016/0042-6822(90)90053-T 2294647 0042-6822 Centrifugation of Tacaribe arenavirus-infected cell extracts on CsCl density gradients was used to separate genomes and antigenomes, which band at 1.31 g/ml as nucleocapsids, from mRNAs which pellet. Primer extensions on the banded RNAs showed that the 5' ends of the genomes and antigenomes were unique, whereas primer extensions on the mRNAs showed that their 5' ends were heterogenous in length, extending 0-4 bases beyond the 3' ends of the templates for their synthesis. This suggests that arenavirus mRNAs may initiate by a cap-snatching mechanism, somewhat similar to influenza viruses and bunyaviruses. We also found an extra G residue at the 5' end of the genome RNA, which was not predicted according to current models. This is now the third time that the unexpected G residue has been found at the 5' end of arenavirus genomes. Animals Arenaviridae/genetics Base Sequence Blotting, Northern Centrifugation, Density Gradient Molecular Sequence Data Oligonucleotide Probes RNA, Messenger/biosynthesis/genetics RNA, Viral/biosynthesis/genetics Templates, Genetic Vero Cells Dewey 616 54 Département de microbiologie et médecine moléculaire 21B false 216 Virologie moléculaire 275 Virology 174 1 b295f763-60ad-4e12-8f0b-1bf32369d93a Article (Published version) Restricted Virology_1990-174-1-53.pdf 3760684 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Nontemplated bases at the 5' ends of Tacaribe virus mRNAs	Virology			1990	654	1

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Hacker, David Lynn