ECM degradation in the Drosophila abdominal epidermis initiates tissue growth that ceases with rapid cell-cycle exit

Article Article scientifique Article ECM degradation in the Drosophila abdominal epidermis initiates tissue growth that ceases with rapid cell-cycle exit eng 2022-03 author Davis John Robert 0000-0002-6022-0369 author Ainslie Anna P. author Williamson John J. author Ferreira Ana 412534 author Torres-Sánchez Alejandro author Hoppe Andreas author Mangione Federica 0000-0003-2838-3536 author Smith Matthew 704700 author Martin-Blanco Enrique 0000-0002-6926-910X author Salbreux Guillaume 0000-0001-7041-1292 947792 Département de génétique et évolution (Genev) (BIANI) author Tapon Nicolas 1285-1300.e4 10.1016/j.cub.2022.01.045 35167804 0960-9822 PMC8967408 https://linkinghub.elsevier.com/retrieve/pii/S0960982222001075 During development, multicellular organisms undergo stereotypical patterns of tissue growth in space and time. How developmental growth is orchestrated remains unclear, largely due to the difficulty of observing and quantitating this process in a living organism. Drosophila histoblast nests are small clusters of progenitor epithelial cells that undergo extensive growth to give rise to the adult abdominal epidermis and are amenable to live imaging. Our quantitative analysis of histoblast proliferation and tissue mechanics reveals that tissue growth is driven by cell divisions initiated through basal extracellular matrix degradation by matrix metalloproteases secreted by the neighboring larval epidermal cells. Laser ablations and computational simulations show that tissue mechanical tension does not decrease as the histoblasts fill the abdominal epidermal surface. During tissue growth, the histoblasts display oscillatory cell division rates until growth termination occurs through the rapid emergence of G0/G1 arrested cells, rather than a gradual increase in cell-cycle time as observed in other systems such as the Drosophila wing and mouse postnatal epidermis. Different developing tissues can therefore achieve their final size using distinct growth termination strategies. Thus, adult abdominal epidermal development is characterized by changes in the tissue microenvironment and a rapid exit from the cell cycle. Drosophila Cell division Development Extracellular matrix Growth control Mechanics Quantitative biology Simulations Dewey 590 Cancer Research UK 17064 The Francis Crick Institute 10317 Wellcome Trust 201358/Z/16/Z Versus Arthritis FC001317 Medical Research Council FC001175 44 Département de génétique et évolution 162 false Current biology 32 6 08b4f483-5d98-42df-972c-2a2c8083baac Article (Published version) Public Article-scientifique-from-unpaywall.pdf 13513162 application/pdf CC BY-4.0 295ba6c01c9332363fcda05c4ba6f7c66291a9497de45887f3172c57745d72d7 openAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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ECM degradation in the Drosophila abdominal epidermis initiates tissue growth that ceases with rapid cell-cycle exit	Current biology			2022	82	61

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