Generation and maintenance of kidney and kidney cancer organoids from patient-derived material for drug development and precision oncology

Article Article scientifique Article Generation and maintenance of kidney and kidney cancer organoids from patient-derived material for drug development and precision oncology eng 2024-11-05 2024-12-12 author Gubala Jakub Molecular Pharmacology Group, School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva, Switzerland.,Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva, Switzerland.,Translational Research Center in Oncohaematology, 1211 Geneva, Switzerland. 409064 Section des sciences pharmaceutiques (FARMA) author Mieville Valentin 0009-0003-4626-0377 Molecular Pharmacology Group, School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva, Switzerland.,Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva, Switzerland.,Translational Research Center in Oncohaematology, 1211 Geneva, Switzerland. 855454 Section des sciences pharmaceutiques (FARMA) author Benamran Daniel Alexandre Israel Division of Urology, Geneva University Hospitals, Geneva, Switzerland. 618957 Département de chirurgie (CHIRU) author Tille Jean-Christophe Division of Pathology, Geneva University Hospitals, Geneva, Switzerland. 87627 author Valerio Massimo Division of Urology, Geneva University Hospitals, Geneva, Switzerland. 405547 Département de chirurgie (CHIRU) author Nowak-Sliwinska Patrycja Molecular Pharmacology Group, School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva, Switzerland.,Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva, Switzerland.,Translational Research Center in Oncohaematology, 1211 Geneva, Switzerland. 848409 Section des sciences pharmaceutiques (FARMA) 101368 10.1016/j.omtm.2024.101368 39659758 2329-0501 PMC11629258 https://www.sciencedirect.com/science/article/pii/S2329050124001840 Despite significant advancements in targeted- and immunotherapies, millions of patients with cancer still succumb to the disease each year. In renal cell carcinoma, up to 25% of metastatic patients do not respond to first-line therapies. This reality underscores the urgent need for innovative or repurposed therapies to effectively treat these patients. Patient-derived organoids represent a promising model for evaluating treatment efficacy and toxicity, offering a potential breakthrough in personalized medicine. However, utilizing organoid models for drug screening presents several challenges. Our protocol aims to address these obstacles by outlining a practical approach to successfully isolate and cultivate patient-derived renal cell carcinoma and kidney organoids for treatment screening purposes. Drug screening Isolation procedure Kidney Patient-derived organoids Precision oncology Renal cell carcinoma Toxicology Dewey 615 Dewey 616.07 Dewey 616 137 Département de pathologie et immunologie 21F 31 Section des sciences pharmaceutiques 17 56 Département de médecine 221 false 391 Métastases du foie 657 1325 Pharmacologie moléculaire Molecular therapy. Methods & clinical development 32 4 381abdeb-9e83-4ad6-b79a-24b4244e5bbd Article (Published version) Public Article_scientifique_from_europePmc.pdf 2964212 application/pdf CC BY-4.0 22adc4de4a44a14fb9a75259b4a427d110c99f479203fc0a17660a203ae81ad6 9d74710e-e847-4563-b179-de4411d223b7 Appendix Public mmc1.pdf 3644482 application/pdf 684458396e36f98008fe605510f17ea7e9cd619b53c254d2a1cc7fed38c66dcd f6e3c942-95cc-441f-8c28-29d514899ad9 Appendix Public mmc2.pdf 6605323 application/pdf 97322445f0136ad272977ad50c3523580587d22420bcef74b7e4050aeb30f935 openAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté des sciences / Section des sciences pharmaceutiques Faculté de médecine / Section de médecine clinique / Département de médecine

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Generation and maintenance of kidney and kidney cancer organoids from patient-derived material for drug development and precision oncology	Molecular therapy. Methods & clinical development			2024	53	31

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Gubala, Jakub