Human primary cells can tell body time: Dedicated to Steven A. Brown

Article Article scientifique Revue de la littérature Human primary cells can tell body time: Dedicated to Steven A. Brown eng 2024-07-01 author Katsioudi Georgia 0000-0001-7007-9832 Department of Surgery, Division of Thoracic and Endocrine Surgery, University Hospitals of Geneva, Geneva, Switzerland.,Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland.,Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland.,Institute of Genetics and Genomics of Geneva (iGE3), Geneva, Switzerland. 403129 Département de chirurgie (CHIRU) author Biancolin Andrew 0000-0003-1419-4881 Department of Surgery, Division of Thoracic and Endocrine Surgery, University Hospitals of Geneva, Geneva, Switzerland.,Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland.,Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland.,Institute of Genetics and Genomics of Geneva (iGE3), Geneva, Switzerland. 402571 Département de chirurgie (CHIRU) author Jiménez-Sánchez Cecilia Department of Surgery, Division of Thoracic and Endocrine Surgery, University Hospitals of Geneva, Geneva, Switzerland.,Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland.,Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland.,Institute of Genetics and Genomics of Geneva (iGE3), Geneva, Switzerland. 893007 author Dibner Charna 0000-0002-4188-803X Department of Surgery, Division of Thoracic and Endocrine Surgery, University Hospitals of Geneva, Geneva, Switzerland.,Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland.,Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland.,Institute of Genetics and Genomics of Geneva (iGE3), Geneva, Switzerland. 583541 Département de chirurgie (CHIRU) 3946-3960 10.1111/ejn.16453 38951126 0953-816X https://onlinelibrary.wiley.com/doi/10.1111/ejn.16453 The field of chronobiology has advanced significantly since ancient observations of natural rhythms. The intricate molecular architecture of circadian clocks, their hierarchical organization within the mammalian body, and their pivotal roles in organ physiology highlight the complexity and significance of these internal timekeeping mechanisms. In humans, circadian phenotypes exhibit considerable variability among individuals and throughout the individual's lifespan. A fundamental challenge in mechanistic studies of human chronobiology arises from the difficulty of conducting serial sampling from most organs. The concept of studying circadian clocks in vitro relies on the groundbreaking discovery by Ueli Schibler and colleagues that nearly every cell in the body harbours autonomous molecular oscillators. The advent of circadian bioluminescent reporters has provided a new perspective for this approach, enabling high-resolution continuous measurements of cell-autonomous clocks in cultured cells, following in vitro synchronization pulse. The work by Steven A. Brown has provided compelling evidence that clock characteristics assessed in primary mouse and human skin fibroblasts cultured in vitro represent a reliable estimation of internal clock properties in vivo. The in vitro approach for studying molecular human clocks in cultured explants and primary cells, pioneered by Steve Brown, represents an invaluable tool for assessing inter-individual differences in circadian characteristics alongside comprehensive genetic, biochemical and functional analyses. In a broader context, this reliable and minimally invasive approach offers a unique perspective for unravelling the functional inputs and outputs of oscillators operative in nearly any human tissue in physiological contexts and across various pathologies. Steven A. Brown Circadian bioluminescence Circadian clockwork Human diseases Human primary skin fibroblasts Humans Circadian Clocks / physiology Animals Circadian Rhythm / physiology History, 21st Century History, 20th Century Cells, Cultured Dewey 612 Dewey 616 Dewey 617 Dewey 576.5 Swiss National Science Foundation Roles of the pancreatic islet cell-specific circadian oscillators in metabolic regulation in physiology and type 2 diabetes 184708 CH Projects Swiss National Science Foundation a- and ß-cellular clocks: a network of functional outputs and their potential therapeutic applications for type 2 diabetes 219187 CH Projects 57 Département de chirurgie 222 134 Département de physiologie cellulaire et métabolisme 21C 56 Département de médecine 221 135 Département de médecine génétique et développement 21D false 913 Endocrinologie et horloge circadienne 948 EJN. European journal of neuroscience 60 2 8bfc0a1f-7771-476f-b986-089f638f8023 Article (Published version) Public Eur_J_of_Neuroscience_-_2024_-_Kats.pdf 1342537 application/pdf CC BY-NC-ND-4.0 566fc2ba28752aa8c7da75ace3b026e67ab5b55dfdd31a9cb40950c921208668 openAccess Faculté de médecine / Section de médecine clinique / Département de chirurgie Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement

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Human primary cells can tell body time: Dedicated to Steven A. Brown	EJN. European journal of neuroscience			2024	59	73

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