Platelet-rich plasma promotes cellular recovery from nicotine-induced toxicity via autophagy modulation

Article Article scientifique Article Platelet-rich plasma promotes cellular recovery from nicotine-induced toxicity via autophagy modulation eng 2026-02-09 2026-02-09 author Veriepe Julie 402429 Section des sciences pharmaceutiques (FARMA) author Cancela José Antonio 507370 CUMD - Div. médecine dentaire régénérative & parodontologie - Communs (DPAR) author Vischer Solange France Chantal 28112 author Turzi Antoine author Cuendet Muriel 0000-0002-1523-1907 686755 Section des sciences pharmaceutiques (FARMA) author Giannopoulou Catherine 23966 CUMD - Div. médecine dentaire régénérative & parodontologie - Communs (DPAR) author Berndt Sarah 812028 7979 10.1038/s41598-026-38188-1 41663725 2045-2322 PMC12957464 https://www.nature.com/articles/s41598-026-38188-1 Chronic exposure to nicotine significantly exacerbates periodontitis, a prevalent inflammatory disease, by inducing cellular processes such as autophagy and inflammation in gingival fibroblasts. Current therapies often fail to fully address these cellular alterations in smokers, highlighting a need for innovative therapeutic and regenerative approaches. This study explores the therapeutic potential of Platelet-Rich Plasma (PRP), a blood-derived product, to modulate nicotine-induced biological activities in primary gingival fibroblasts, particularly in the case of periodontitis in smokers. Gingival fibroblasts were treated with increasing concentrations of nicotine, which led to senescence and autophagy. Nicotine at high concentrations triggered cellular vacuolization, and a decrease in metabolism, viability and proliferation. Concomitant cell treatment with 10% PRP reversed nicotine effects and significantly increased cell migration potential. In Caenorhabditis elegans, PRP reduced the nicotine-induced autophagic activity. A screening of the gingival fibroblast secretome revealed a modulation of autophagy-related cytokines in response to nicotine and/or PRP. The findings demonstrate that PRP could effectively inhibit nicotine-induced autophagy in gingival fibroblasts, offering insights into its possible use as a therapeutic tool for managing periodontitis in smokers. The study underscores the potential of PRP in altering disease progression by modulating key cellular processes affected by smoking. Autophagy Nicotine Periodontitis Platelet rich plasma Dewey 617.6 Dewey 615 206 Division de paradontologie 24P2 31 Section des sciences pharmaceutiques 17 false 884 Pharmacognosie 360 Groupe Giannopoulou Catherine (médecine dentaire) 626 Scientific reports 16 1 b012e89b-f0c2-404b-adf3-0bbe842a950f Article (Published version) Public Platelet-rich_plasma_promotes_cellu.pdf 6310521 application/pdf CC BY-4.0 bc7deb1a22bcedf1781c2842a2ee8937cadef286c0d56edf5f9dc3eb013ab498 openAccess Faculté de médecine / Section de médecine dentaire / Département de médecine dentaire préventive et de premier recours / Division de paradontologie Faculté des sciences / Section des sciences pharmaceutiques

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Platelet-rich plasma promotes cellular recovery from nicotine-induced toxicity via autophagy modulation	Scientific reports			2026	9	15

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Veriepe, Julie