Selective plasticity of callosal neurons in the adult contralesional cortex following murine traumatic brain injury

Article Article scientifique Article Selective plasticity of callosal neurons in the adult contralesional cortex following murine traumatic brain injury eng 2022-05-12 2022-05-12 author Empl Laura author Chovsepian Alexandra author Chahin Maryam author Kan Wing Yin Vanessa author Fourneau Julie author Van Steenbergen Valérie 0000-0002-5836-2122 author Weidinger Sanofer author Marcantoni Maite 0000-0001-6665-5251 author Ghanem Alexander author Bradley Peter Edward 40240 author Conzelmann Karl Klaus 0000-0002-8614-3656 author Cai Ruiyao author Ghasemigharagoz Alireza author Ertürk Ali author Wagner Ingrid 0000-0003-4437-9799 604950 author Kreutzfeldt Mario 736806 author Merkler Doron 0000-0002-0247-2007 735735 author Liebscher Sabine 0000-0001-5633-8981 author Bareyre Florence M 0000-0002-0917-1725 2659 10.1038/s41467-022-29992-0 35551446 2041-1723 PMC9098892 Traumatic brain injury (TBI) results in deficits that are often followed by recovery. The contralesional cortex can contribute to this process but how distinct contralesional neurons and circuits respond to injury remains to be determined. To unravel adaptations in the contralesional cortex, we used chronic in vivo two-photon imaging. We observed a general decrease in spine density with concomitant changes in spine dynamics over time. With retrograde co-labeling techniques, we showed that callosal neurons are uniquely affected by and responsive to TBI. To elucidate circuit connectivity, we used monosynaptic rabies tracing, clearing techniques and histology. We demonstrate that contralesional callosal neurons adapt their input circuitry by strengthening ipsilateral connections from pre-connected areas. Finally, functional in vivo two-photon imaging demonstrates that the restoration of pre-synaptic circuitry parallels the restoration of callosal activity patterns. Taken together our study thus delineates how callosal neurons structurally and functionally adapt following a contralateral murine TBI. Animals Brain Injuries, Traumatic Cerebral Cortex Corpus Callosum / physiology Mice Neurons / physiology Dewey 616.07 137 Département de pathologie et immunologie 21F false 565 La Sclérose en plaques 908 https://zenodo.org/record/6364730#.Y9ebQ4SZOMo Nature communications 13 1 943e307f-0bde-46ba-ac3e-65ee740b6568 Article (Published version) Public Article-scientifique-from-europePmc.pdf 6424326 application/pdf CC BY-4.0 6aea527c-881f-4072-913d-80c94e71957b Appendix Public 41467_2022_29992_MOESM2_ESM.pdf 385140 application/pdf 662d9274-0b78-4bf5-afea-17d745145c03 Appendix Public 41467_2022_29992_MOESM1_ESM.pdf 656796 application/pdf 7447997e-20e8-4f09-b4e4-b7de8733ceb3 Appendix Public 41467_2022_29992_MOESM3_ESM.pdf 3591481 application/pdf f2bc6c26-e107-4f4c-8213-23f031e5af65 Appendix Public 41467_2022_29992_MOESM4_ESM.pdf 32865 application/pdf 7f6b926c-3f44-41b8-b55f-8b5631466903 Appendix Public 41467_2022_29992_MOESM5_ESM.avi 3110136 video/x-msvideo openAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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Selective plasticity of callosal neurons in the adult contralesional cortex following murine traumatic brain injury	Nature communications			2022	162	209

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Bradley, Peter Edward