A novel biocompatible hyaluronic acid-chitosan hybrid hydrogel for osteoarthrosis therapy

Article Article scientifique Article A novel biocompatible hyaluronic acid-chitosan hybrid hydrogel for osteoarthrosis therapy eng 2015 author Kaderli Sema Gwendolyn 595492 author Boulocher C author Pillet Eric François Alfred 38165 author Watrelot-Virieux D author Rougemont-Pidoux Anne-Laure 29140 author Roger T author Viguier Emilie Jennifer 527753 author Gurny Robert 24779 author Scapozza Leonardo 600318 author Jordan Olivier 0000-0003-3752-2198 133859 158-168 10.1016/j.ijpharm.2015.01.052 25666331 0378-5173 A conventional therapy for the treatment of osteoarthrosis is intra-articular injection of hyaluronic acid, which requires repeated, frequent injections. To extend the viscosupplementation effect of hyaluronic acid, we propose to associate it with another biopolymer in the form of a hybrid hydrogel. Chitosan was chosen because of its structural similarity to synovial glycosaminoglycans, its anti-inflammatory effects and its ability to promote cartilage growth. To avoid polyelectrolyte aggregation and obtain transparent, homogeneous gels, chitosan was reacetylated to a 50% degree, and different salts and formulation buffers were investigated. The biocompatibility of the hybrid gels was tested in vitro on human arthrosic synoviocytes, and in vivo assessments were made 1 week after subcutaneous injection in rats and 1 month after intra-articular injection in rabbits. Hyaluronic acid-chitosan polyelectrolyte complexes were prevented by cationic complexation of the negative charges of hyaluronic acid. The different salts tested were found to alter the viscosity and thermal degradation of the gels. Good biocompatibility was observed in rats, although the calcium-containing formulation induced calcium deposits after 1 week. The sodium chloride formulation was further tested in rabbits and did not show acute clinical signs of pain or inflammation. Hybrid HA-Cs hydrogels may be a valuable alternative viscosupplementation agent. Dewey 615 Dewey 616.07 31 Section des sciences pharmaceutiques 17 137 Département de pathologie et immunologie 21F false 391 Métastases du foie 657 International journal of pharmaceutics 483 1-2 6e8ef78d-dac2-4f5f-a5be-444d48603fa9 Article (Accepted version) Restricted Rougemont AL Int J Pharma 2015,483,158.pdf 2274185 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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A novel biocompatible hyaluronic acid-chitosan hybrid hydrogel for osteoarthrosis therapy	International journal of pharmaceutics			2015	911	2

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Pillet, Eric François Alfred