Heterogeneity of polyoma tumors in hamsters: analysis of cytoskeletal proteins and viral gene expression

Article Article scientifique Article Heterogeneity of polyoma tumors in hamsters: analysis of cytoskeletal proteins and viral gene expression eng 1990 author Combette Jean-Marc 37500 author Sappino Pascal 22712 author Turler Hans 25123 521-528 10.1002/ijc.2910450325 2155184 0020-7136 Murine polyomavirus-induced hamster tumors revealed an unexpected heterogeneity with respect to patterns of cytoskeletal proteins expressed in different visceral and subcutaneous tumors and with respect to viral gene expression early during tumor outgrowth. All tumors analyzed expressed vimentin. Desmin was found in all heart tumors, to variable degrees in kidney tumors and in trace amounts only in 1 out of 4 s.c. tumors. The alpha-smooth-muscle actin isoform was observed in heart tumors only and was restricted to structures that we interpret as being proliferating pericytes or proliferating smooth-muscle cells of the media. In kidneys of infected newborn animals and before the appearance of macroscopic tumors, viral early mRNAs were transcribed from free viral genomes. In tumor tissue the size of the viral transcripts was altered, suggesting that they were transcribed from integrated viral DNA. Since in each tumor discrete bands of viral RNAs were detected, individual tumors arose presumably from single cells and one functional integration event. In contrast, in situ hybridizations of kidney tissue and tumors showed large quantitative differences in viral gene expression not only between different tumors but also between individual cells of the same tumor. Animals Newborn animals Cricetinae Desmin/analysis Heart Neoplasms/analysis Kidney neoplasms/analysis Mesocricetus Polyomavirus Messenger RNA/analysis Viral RNA/analysis Skin neoplasms/analysis Tumor virus infections Vimentin/analysis Dewey 570 43 Département de biologie moléculaire 161 false International Journal of Cancer 45 3 e2b13871-b4c1-4921-9a23-e33339f2b60d Article (Published version) Restricted combette_IJC_199045521.pdf 1367651 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Heterogeneity of polyoma tumors in hamsters: analysis of cytoskeletal proteins and viral gene expression	International Journal of Cancer			1990	369	0

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Combette, Jean-Marc