Mutations in Rous Sarcoma Virus Nucleocapsid Protein p12 (NC): Deletions of Cys-His Boxes

Article Article scientifique Article Mutations in Rous Sarcoma Virus Nucleocapsid Protein p12 (NC): Deletions of Cys-His Boxes eng 1988 author Meric Claude 68095 author Gouilloud Evelyne Patricia M. 33033 author Spahr Pierre-François 25043 3328-3333 10.1128/JVI.62.9.3328-3333.1988 2841485 0022-538X Rous sarcoma virus nucleocapsid protein p12 (NC) contains two conserved amino acid motifs, the Cys-His boxes, which constitute potential metal-binding domains. To try to understand the function of NC and of each of its Cys-His boxes during the viral life cycle, particularly in viral RNA packaging, we have used synthetic oligonucleotides to delete precisely either the proximal or the distal box, or both Cys-His boxes. The mutant DNAs were transfected into chicken embryo fibroblasts, and the virions produced in a transient assay were characterized biochemically for production of viral proteins and particles, RNA packaging, and infectivity. The results indicated the following. (i) The deletion of either the proximal or the distal box decreases the amount of viral RNA packaged in the particles and results in incomplete 70S dimer formation. (ii) The deletion of both boxes inhibits viral RNA packaging. (iii) The deletion of the proximal, but not the distal, box suppresses any detectable infectivity, while the deletion of the distal, but not the proximal, box lowers infectivity 100 to 200 times. Animals Avian sarcoma viruses/genetics Base sequence Capsid/genetics Cultured cells Chick embryo Viral DNA/genetics Fibroblasts Mutation Viral RNA/genetics Transfection Viral core proteins/genetics Dewey 570 43 Département de biologie moléculaire 161 false Journal of Virology 62 9 15eb6158-32c5-4f9b-b38d-ec47ecc4ade4 Article (Published version) Restricted jvirol00088-0266.pdf 1720758 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Mutations in Rous Sarcoma Virus Nucleocapsid Protein p12 (NC): Deletions of Cys-His Boxes	Journal of Virology			1988	286	0

Archive ouverte UNIGE

Gouilloud, Evelyne Patricia M.