Néoglycoprotéines à sonde paramagnétique

Article Article scientifique Article Néoglycoprotéines à sonde paramagnétique fre 1992 author Tronchet Jean Marcel Julien 25054 Administration de la faculté des sciences (SIAD) author Buchholzer Franz Joséf 31027 author Zsely Schaffter Martina Maria 24230 author Geoffroy Michel 25153 Administration de la faculté des sciences (SIAD) author Schmidt M. author Mansi M.N. 2-8 0031-6865 1-Deoxy-1(N-hydroxyamino)-D-glucitol has been fixed onto human and bovine serumalbumin through a pentamethylene spacer arm. The modified proteins were purified by dialysis, electrophoresis and high pressure liquid chromatography to insure, in particular, a complete removal of low molecular weight starting materials. The fixation of the sugar derivative onto the protein was established by an increase in sugar content and the obtention of usable e.s.r. spectra from the nitroxide free radicals, obtained from the spontaneous oxidation of the N-hydroxyamino group. E.s. r. hyperfine coupling constants gave information on the conformation of the magnetic probe. Dewey 540 Dewey 615 38 Département de chimie physique 142 31 Section des sciences pharmaceutiques 17 false Pharmaceutica Acta Helvetiae 2 21ddf13b-1631-413d-9a98-fd04fd863e87 Article (Published version) Public Tronchet_PAH_199222.pdf 531500 application/pdf 1aa59dc2231ed8aecc8052bbdb6c52d8b2ce964943874c61971aeafd1036d05e openAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie physique Faculté des sciences / Section des sciences pharmaceutiques

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Néoglycoprotéines à sonde paramagnétique	Pharmaceutica Acta Helvetiae			1992	70	48

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Buchholzer, Franz Joséf