Identification of a neuronal calcium sensor (NCS-1) possibly involved in the regulation of receptor phosphorylation

Article Article scientifique Article Identification of a neuronal calcium sensor (NCS-1) possibly involved in the regulation of receptor phosphorylation eng 1995 author Nef Serge 0000-0001-5462-0676 29062 author Fiumelli Hubert 30207 author De Castro Eduardo 28647 author Raest M.-B. author Nef Patrick 22181 365-378 10.3109/10799899509045227 8903951 1079-9893 https://www.tandfonline.com/doi/full/10.3109/10799899509045227 Persistent stimulation of G protein-coupled receptors by agonists leads rapidly to reduced responses, a phenomenon described as desensitization. It involves primarily the phosphorylation of receptor sites by specific kinases of the G protein-coupled receptor kinase (GRK) family. The β-adrenergic receptor kinase 1 (GRK2) desensitizes agonist-activated β2-adrenergic receptors, whereas rhodopsin kinase (GRK1) phosphorylates and inactivates photon-activated rhodopsin. Little is known about the role of calcium in desensitization. Here we report the characterization of a novel neuronal calcium sensor (NCS) named NCS-1 possibly involved in the regulation of receptor phosphorylation. NCS-1 is a new member of the EF-hand superfamily, which includes calmodulin, troponin C, parvalbumin, and recoverins. By Northern analysis and in situ hybridization, we discovered that NCS-1 is specifically expressed in the central and peripheral nervous systems. Chick NCS-1 has 72% of amino acid identity with Drosophila frequenin, a protein found in the nervous system and at the motor nerve terminals of neuromuscular junctions. By analogy with the reported function for two other members of the NCS family, we discuss whether G protein-coupled receptors or GRKs are the targets of neuronal calcium sensors. Dewey 540 39 Département de biochimie 144 false Journal of receptor and signal transduction research 15 1-4 9d95dc9a-f07b-4dab-a203-083d898562a7 Article (Published version) Restricted Nef_JRSTR_199515365.pdf 5483306 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Identification of a neuronal calcium sensor (NCS-1) possibly involved in the regulation of receptor phosphorylation	Journal of receptor and signal transduction research			1995	137	0

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Fiumelli, Hubert