HLA-DPB1 DNA polymorphism in the Swiss population : linkage disequilibrium with other HLA loci and population genetics affinities

Article Article scientifique Article HLA-DPB1 DNA polymorphism in the Swiss population : linkage disequilibrium with other HLA loci and population genetics affinities eng 1994 author Grundschober Christophe 30022 author Sanchez-Mazas Alicia 22545 author Excoffier Laurent Georges Louis 22395 author Langaney André 25252 author Jeannet Michel 25296 author Tiercy Jean-Marie 26423 143-157 0960-7420 Allelic diversity at the HLA-OPBl locus was determined by PCR-oligotyping in a sample of 125 healthy Swiss individuals. A total of 17 alleles were detected among which four main alleles (DPBP1*0401, *0201, *0301, *0402) reached a cumulative frequency 0f 74.8%. HLA-A and -B (by serology) and HLA-DRBl (by oligotyping) allelic pOlymorphisms were analysed also. HLA-B and HLA-DRBl loci were highly polymorphic with 25 and 28 alleles respectively and similar heterozygosity levels of 0.93 and 0.92. These two loci were found to be more polymorphic than expected under neutrality, while lower heterozygosity levels were found for HLA-A (0.87) and DPB 1 (0.81) loci. This paper presents also a global comparison of DPBl allelic frequencies among 15 populations from four continents. As opposed to the DRBl locus, overall DPB1 is shown to have a lower level of polymorphism and may be considered as neutral in all tested populations. DPB1 genetic diversity is correlated significantly with geography also, as found previously for DRBl. Two- and four-locus haplotype frequencies were determined and the significance of their linkage disequilibrium tested by an original non-parametric method. A significant positive linkage disequilibrium was found for 11 A-B, 16 B-DRB1, 7 DRBI-DPBl and 3 A-B-DRBI-DPBl haplotypes. The overall linkage disequilibrium between DRB1 and DPB1 was much lower than expected from the physical distance and lower than for A-B and B-DRB 1 pairs. The implications of these results for bone marrow transplantation and for the evolution of HLA loci are discussed. Génétique humaine Suisse Polymorphisme génétique HLA Dewey 599.9 Dewey 590 44 Département de génétique et évolution 162 140 Département d'anthropologie et écologie 164 false 1056 Unité d'anthropologie European journal of immunogenetics 21 bfb5a77b-921e-439b-b7fb-1342267c288a Article (Published version) Public Grundschober ASM EJI 1994.pdf 610991 application/pdf openAccess Faculté des sciences / Section de biologie / Département de génétique et évolution Faculté des sciences / Section de biologie / Département d'anthropologie et écologie

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HLA-DPB1 DNA polymorphism in the Swiss population : linkage disequilibrium with other HLA loci and population genetics affinities	European journal of immunogenetics			1994	867	159

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Grundschober, Christophe