Partial characterization of a Sendai virus replication promoter and the rule of six

Article Article scientifique Article Partial characterization of a Sendai virus replication promoter and the rule of six eng 1996 author Pelet Thierry 26893 author Delenda Christophe 100594 author Gubbay Oliver James 29827 author Garcin Dominique 24296 author Kolakofsky Daniel 25420 405-414 10.1006/viro.1996.0547 8874501 0042-6822 We have used a cDNA copy of a natural, internally deleted, Sendai virus defective interfering genome to study the effect of insertions and deletions (which maintain the hexamer genome length) on the ability of viral genomes to be amplified in a transfected cell system. The insertion of 18 nt at nt72 (In the 5' untranslated region of the N gene, just downstream of the le+ region) was found to be lethal, whereas similar insertions further from the genome ends were well tolerated. Curiously, the insertion of 6 nt on either side of the le+/N junction (at nt47 and nt87) was well tolerated, but the insertion of 12 nt at either site, or of 6 nt at both sites, largely ablated genome amplification. These results suggest that an element of this replication promoter is located downstream of nt87, in the 5' untranslated region of the first gene. Remarkably, the addition of 6 nt by the insertion of 2, 3, or 4 nt at nt47 plus the insertion of 4, 3, or 2 nt, respectively, at nt87 was poorly tolerated, presumably because the hexamer phase of the intervening sequence was altered with respect to the N subunits of the template. These results suggest that the rule of six operates, at least in part, at the level of the initiation of antigenome synthesis. Genome, Viral Humans Mutagenesis, Insertional Promoter Regions, Genetic Respirovirus/genetics/physiology Sequence Deletion Tumor Cells, Cultured Virus Replication/genetics Dewey 616 54 Département de microbiologie et médecine moléculaire 21B false 216 Virologie moléculaire 275 Virology 224 2 83d983af-9c56-43eb-97d2-3af827fb09ff Article (Published version) Public 1-s2.0-S0042682296905474-main.pdf 1092598 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Partial characterization of a Sendai virus replication promoter and the rule of six	Virology			1996	657	465

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Gubbay, Oliver James