SARs stimulate but do not confer position independent gene expression

Article Article scientifique Article SARs stimulate but do not confer position independent gene expression eng 1994 author Poljak-Carpousis Leonora Gabriela 23369 author Seum Carole 22058 author Mattioni Duval Tiziana 28489 author Laemmli Ulrich Karl 25441 4386-4394 10.1093/nar/22.21.4386 0305-1048 https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/22.21.4386 Two minimal scaffold-associated regions (SARs) from Drosophila were tested in stably transformed cells for their effects on the expression of reporter genes. The expression of genes bounded by two SARs is consistently stimulated by about 20- to 40-fold, if the average of a pool of cell transformants is analyzed. However, analysis of individual, stable cell transformants demonstrates that flanking SAR elements do not confer position-independent expression on the reporter gene and that the extent of position-dependent variegation is similarly large with or without the flanking SAR elements. The SAR stimulation of expression is observed in stable but not in transiently transfected cell lines. The Drosophila scs and scs' boundary elements, which do not bind to the nuclear matrix in vitro, are only about one-tenth as active as SARs in stimulating expression in stable transformants. Interestingly, the SAR stimulatory effect can be blocked by a fragment containing CpG islands (~˜70% GC), if positioned between the SAR and the enhancer. In contrast, when inserted in the same position, control fragments, such as the scs/scs' elements, do not interfere with SAR function. Dewey 540 Dewey 570 39 Département de biochimie 144 43 Département de biologie moléculaire 161 false Nucleic Acids Research 22 21 fe91b45c-49bd-4980-ac43-1dd99bd3ea83 Article (Published version) Public 22-21-4386.pdf 3687598 application/pdf CC BY openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de biologie / Département de biologie moléculaire

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SARs stimulate but do not confer position independent gene expression	Nucleic Acids Research			1994	325	275

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Mattioni Duval, Tiziana