Splicing factor SF1 from Drosophila and Caenorhabditis: Presence of an N-terminal RS domain and requirement for viability

Article Article scientifique Article Splicing factor SF1 from Drosophila and Caenorhabditis: Presence of an N-terminal RS domain and requirement for viability eng 1999-12 author Mazroui Rachid 28480 author Puoti Alessandro author Kraemer Angela 22808 Département de biologie moléculaire et cellulaire (MOCEL) 1615-1631 10.1017/S1355838299991872 10606272 1355-8382 PMC1369883 Splicing factor SF1 contributes to the recognition of the 3' splice site by interacting with U2AF65 and binding to the intron branch site during the formation of the early splicing complex E. These interactions and the essential functional domains of SF1 are highly conserved in Saccharomyces cerevisiae. We have isolated cDNAs encoding SF1 from Drosophila (Dm) and Caenorhabditis (Ce). The encoded proteins share the U2AF65 interaction domain, a hnRNP K homology domain, and one or two zinc knuckles required for RNA binding as well as Pro-rich C-terminal sequences with their yeast and mammalian counterparts. In contrast to SF1 in other species, DmSF1 and CeSF1 are characterized by an N-terminal region enriched in Ser, Arg, Lys, and Asp residues with homology to the RS domains of several splicing proteins. These domains mediate protein–protein or protein–RNA interactions, suggesting an additional role for DmSF1 and CeSF1 in pre-mRNA splicing. Human (Hs), fly, and worm SF1 interact equally well with HsU2AF65 or the Drosophila homolog DmU2AF50. Moreover, DmSF1 lacking its N terminus is functional in prespliceosome formation in a HeLa splicing system, emphasizing the conserved nature of interactions at an early step in spliceosome assembly. The Ce-SF1 gene is located in a polycistronic transcription unit downstream of the genes encoding U2AF35 (uaf-2) and a cyclophilin (cyp-13), implying the coordinate transcriptional regulation of these genes. Injection of double-stranded RNA into C. elegans results in embryonic lethality; thus, the SF1 gene is essential not only in yeast but also in at least one metazoan. Alternative splicing KH domain Pre-mRNA splicing Protein–protein interaction RNA interference Spliceosome U2AF Dewey 570 46 Département de biologie cellulaire 165 false RNA 5 12 c8533238-9419-4b8b-a282-4cf208b5eb6d Article (Published version) Restricted Mazrouli_RNA_199951615.pdf 917624 application/pdf 80d2144d488f988e4541826835310e9dfd3faf2216621fd5eff6ad0d51ef38bc restrictedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Splicing factor SF1 from Drosophila and Caenorhabditis: Presence of an N-terminal RS domain and requirement for viability	RNA			1999	60	0

Archive ouverte UNIGE

Mazroui, Rachid