Role of macrophage inflammatory peptide-2 in cerulein-induced acute pancreatitis and pancreatitis-associated lung injury

Article Article scientifique Article Role of macrophage inflammatory peptide-2 in cerulein-induced acute pancreatitis and pancreatitis-associated lung injury eng 2003 author Pastor Catherine 0000-0002-1551-3824 128329 author Rubbia-Brandt Laura 31809 author Hadengue Antoine 30244 author Jordan Martha Cacilie 27854 author Morel Philippe 22337 author Frossard Jean-Louis 35038 471-478 10.1097/01.lab.0000063928.91314.9f 12695550 0023-6837 Acute pancreatitis is an inflammatory process of variable severity, and leukocytes are thought to play a key role in the development of pancreatitis and pancreatitis-associated lung injury. The effects of mediators released by these inflammatory cells may induce tissue damage. The aim of our study was to evaluate the role of the chemokine, macrophage inflammatory protein-2 (MIP-2), in the pathogenesis of cerulein-induced pancreatitis and pancreatitis-associated lung injury. The severity of pancreatitis was measured by serum amylase, pancreatic edema, acinar cell necrosis, and myeloperoxidase activity. Lung injury was quantitated by evaluating lung microvascular permeability and lung myeloperoxidase activity. To determine the role of MIP-2 in the pathophysiology of the disease, anti-MIP-2 antibody was administered either 1 hour before or 2 hours after the start of cerulein administration. MIP-2 concentrations increased in serum, pancreas, and lung tissues in mice treated with cerulein. Anti-MIP-2 antibody administrated either before or after cerulein partially protected against pancreas and lung injury. These results show that MIP-2 plays a key role in the pathophysiology of acute pancreatitis and that MIP-2 blockade may improve the outcome of the disease. Acute Disease Amylases/blood Animals Ceruletide/administration & dosage/toxicity Chemokine CXCL2 Disease Models, Animal Edema/chemically induced/pathology Fluorescent Antibody Technique, Indirect Gastrointestinal Agents/administration & dosage/toxicity Injections, Intraperitoneal Lung Diseases/chemically induced/complications/metabolism Male Mice Mice, Inbred Strains Monokines/physiology Necrosis Pancreas/drug effects/pathology Pancreatitis/chemically induced/complications/metabolism Peroxidase/blood Dewey 616 Dewey 616.07 Dewey 617 137 Département de pathologie et immunologie 21F 56 Département de médecine 221 57 Département de chirurgie 222 false 79 Chirurgie viscérale 104 1329 Chirurgie viscérale (HUG) 391 Métastases du foie 657 396 Pharmacologie et imagerie du foie 664 335 Pancréatite aiguë 601 Laboratory investigation 83 4 25af0453-0641-415c-abc9-97716767c03f Article (Published version) Restricted Lab Invest 2003.pdf 1869923 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine clinique / Département de chirurgie

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Role of macrophage inflammatory peptide-2 in cerulein-induced acute pancreatitis and pancreatitis-associated lung injury	Laboratory investigation			2003	828	41

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Jordan, Martha Cacilie