Distribution pattern of three neural calcium-binding proteins (NCS-1, VILIP and recoverin) in chicken, bovine and rat retina

Article Article scientifique Article Distribution pattern of three neural calcium-binding proteins (NCS-1, VILIP and recoverin) in chicken, bovine and rat retina eng 1995-07 author De Raad Saskia 27756 author Comte Michelle 26003 author Nef Patrick 22181 author Lenz Stefan E. author Gundelfinger Eckart D. author Cox Jos Adrian 25544 524-535 10.1007/BF00174325 0018-2214 http://link.springer.com/10.1007/BF00174325 Neural Ca<sup>2+</sup>-binding proteins (NCaPs) constitute a subfamily of 4-EF-hand proteins, and display a histological and structural dichotomy: the A-type NCaPs are selectively expressed by the retina and pineal organ and display two canonical EF-hands, whereas the B-type NCaPs are found in the entire brain and present three regular EF-hands. In this study, antisera were raised against the A-type NCaP recoverin (26 kDa) and the B-type NCaPs VILIP and NCS-1 (22 kDa). Since the sequence identity among NCaPs is high, specific polyclonal antibodies were purified by double cross-immunoaffinity chromatography; both ELISA and immunoblot analyses determined that the resulting antibodies showed selectivity ratios inferior to 1/363 for the two other related NCaPs. Besides, the anti-VILIP antibodies displayed some affinity toward neurocalcin δ, and the antirecoverin antibodies recognized a 24 kDa protein, which is most likely visinin. Thus, immunohistochemical studies on the chicken, rat and cow retina revealed that anti-recoverin antibodies recognized the vertebrate photoreceptors and a small number of mammalian bipolar cells. Anti-VILIP antibodies exclusively labelled the inner Retina, I.e. the amacrine and ganglion cells. NCS-1 was mainly present in the photoreceptor inner segments, the inner plexiform layer and the ganglion cells. NCS-1 showed the highest species disparity. The retinal localization of NCS-1 and VILIP offered an important morphological basis for the understanding of their function. Furthermore, specific antibodies against the NCaPs may enable the identification of cell populations in more complex neural tissues, such as the brain. Retina Ganglion Cell Immunoblot Analysis Neural Tissue Bipolar Cell Dewey 540 Dewey 617 63 Département d'anesthésiologie, pharmacologie, soins intensifs et urgences 229 39 Département de biochimie 144 false Histochemical journal 27 7 b8699fbd-bfcf-4506-8010-f3be96b90603 Article (Published version) Public BF00174325.pdf 1179533 application/pdf OA NATIONAL openAccess Faculté de médecine / Section de médecine clinique / Département d'anesthésiologie, pharmacologie, soins intensifs et urgences Faculté des sciences / Section de chimie et biochimie / Département de biochimie

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Distribution pattern of three neural calcium-binding proteins (NCS-1, VILIP and recoverin) in chicken, bovine and rat retina	Histochemical journal			1995	126	396

Archive ouverte UNIGE

De Raad, Saskia