SARs are <i>cis</i> DNA Elements of Chromosome Dynamics: Synthesis of a SAR Repressor Protein

Article Article scientifique Article SARs are <i>cis</i> DNA Elements of Chromosome Dynamics: Synthesis of a SAR Repressor Protein eng 1995 author Strick Reiner 27051 author Laemmli Ulrich Karl 25441 1137-1148 10.1016/0092-8674(95)90140-X 0092-8674 https://linkinghub.elsevier.com/retrieve/pii/009286749590140X SARs are candidate DNA elements for defining the bases of chromatin loops and possibly for serving as cis elements of chromosome dynamics. SARs contain numerous A tracts, whose altered DNA structure is recognized by cooperatively interacting proteins such as topoisomerase II. We constructed multi-AT hook (MATH) proteins and demonstrate that they specifically bind the clustered A tracts of SARs in chromatin and chromosomes. They are also potent inhibitors of chromosome assembly in mitotic Xenopus extracts, demonstrating the importance of SARs in this process. Titration of SARs with MATH20 (20 hooks) blocks shape determination of chromatids but not chromatin condensation per se. SARs are also required for shape maintenance of chromosomes. If MATH20 is added after formation of chromatids, they collapse and are reshaped by an active, mitotic process into spherical chromatid balls. Dewey 540 Dewey 570 39 Département de biochimie 144 43 Département de biologie moléculaire 161 false Cell 83 7 1777ffeb-9502-430a-9b39-7d13d743fac0 Article (Published version) Restricted 1-s2.0-009286749590140X-main.pdf 5447256 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de biologie / Département de biologie moléculaire

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SARs are cis DNA Elements of Chromosome Dynamics: Synthesis of a SAR Repressor Protein	Cell			1995	241	0

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Strick, Reiner