Uncoupling Protein-3 Expression in Rodent Skeletal Muscle Is Modulated by Food Intake but Not by Changes in Environmental Temperature

Article Article scientifique Article Uncoupling Protein-3 Expression in Rodent Skeletal Muscle Is Modulated by Food Intake but Not by Changes in Environmental Temperature eng 1998-01-02 author Boss Olivier Department of Medical Biochemistry, Faculty of Medicine, University of Geneva, 1 Michel Servet, 1211 Geneva 4, Switzerland. Olivier.Boss@medecine.unige.ch 26723 author Samec-Schnieper Sonia 26924 author Kuhne Françoise 23661 author Bijlenga Philippe Alexandre Pierre 0000-0002-3586-2757 104899 Département des neurosciences cliniques (OTONO) author Assimacopoulos Françoise 26365 author Seydoux Josiane 25038 Décanat et administration de la faculté de médecine (ADMED) author Giacobino Jean-Paul 24897 Décanat et administration de la faculté de médecine (ADMED) author Muzzin Patrick 23003 5-8 10.1074/jbc.273.1.5 9417036 0021-9258 https://linkinghub.elsevier.com/retrieve/pii/S0021925818385363 A new member of the uncoupling protein (UCP) family called UCP3 has recently been cloned and shown to be highly expressed in skeletal muscle of rodents and humans. In the present study, UCP3 was overexpressed in C2C12myoblasts where it acts as an uncoupling protein. Changes in UCP3 mRNA expression were examined in rodent muscles under conditions known to modulate thermogenesis in brown adipose tissue. In skeletal muscle, UCP3 expression did not change in response to 48 h of cold exposure (6 °C), whereas it was decreased by 81% or increased 5.6-fold by 1 week of 50% food restriction or fasting, respectively. It was also decreased by 36% in soleus muscle of obese (fa/fa) as compared with lean Zucker rats. The unexpected rise of UCP3 mRNA level induced by fasting did not change in vitro muscle basal heat production rate but decreased by 31% the capacity to produce heat in response to the uncoupler carbonylcyanidep-trifluoromethoxyphenylhydrazone. This decrease may reflect underlying uncoupling by UCP3. Up-regulation of UCP3 mRNA after a 24-h fast was still observed in mice exposed at thermoneutrality. These results show that the increase in UCP3 expression induced by fasting is associated with the maintenance of thermogenesis measured in muscle in vitro and is not modulated by environmental temperature. The notion that UCP3 expression is modulated by food intake is of importance to better unders Animals Calorimetry Carrier Proteins / genetics Cell Line Female Food Gene Expression Regulation Ion Channels Male Mice Mice, Inbred C57BL Mitochondria, Muscle / metabolism Mitochondrial Proteins Muscle, Skeletal / metabolism RNA, Messenger / genetics Rats Rats, Sprague-Dawley Rats, Zucker Temperature Uncoupling Protein 3 Dewey 612 134 Département de physiologie cellulaire et métabolisme 21C false 513 Groupe Schaller Karl Lothard (neurochirurgie) 851 The Journal of biological chemistry 273 1 9dc42a1b-d0d3-4d05-8463-4b5a4f9d7162 Article (Published version) Public PIIS0021925818385363.pdf 279267 application/pdf CC BY-4.0 65819309141697534e93191cd52f1983c50ea9c68b0770106a54e6faea8e9dc7 openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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Uncoupling Protein-3 Expression in Rodent Skeletal Muscle Is Modulated by Food Intake but Not by Changes in Environmental Temperature	The Journal of biological chemistry			1998	42	73

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Boss, Olivier