Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene

Article Article scientifique Article Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene eng 1998 author Kraemer Angela 22808 author Quentin Mireille 26128 author Mulhauser Frank 22591 29-37 10.1016/S0378-1119(98)00058-4 9573336 0378-1119 Several cDNAs encoding the essential human splicing facor (SF) 1 have been cloned. Comparison of the cDNA sequences suggested that the corresponding mRNAs are generated by alternative splicing from a common pre-mRNA. To confirm this assumption and to analyze possible modes used in the generation of these mRNAs, we have determined the structure of the gene encoding SF1. The gene extends over approximately 15kb and contains 14 exons. The exon/intron structure and sequences at the splice sites are highly conserved in the corresponding mouse gene. The human SF1 gene is located on chromosome 11 close to the gene encoding Menin, recently identified as the gene responsible for multiple endocrine neoplasia-type 1 (MEN1). The absence of a TATA box in the 5' flanking region of the SF1 transcription unit suggests that the SF1 gene represents a housekeeping gene. However, genomic sequence analysis revealed putative binding sites for regulatory transcription factors upstream of the 5' end of the cDNA. Analysis of the SF1 genomic and cDNA sequences predicts the use of duplicated 5' and 3' splice sites as well as exon skipping and intron inclusion to generate six SF1 mRNAs by alternative splicing events. Alternative Splicing/genetics Base Sequence Binding Sites/genetics Chromosome Mapping Chromosomes, Human, Pair 11 Cloning, Molecular DNA-Binding Proteins Exons/genetics Humans Introns/genetics Molecular Sequence Data Multiple Endocrine Neoplasia Type 1/genetics RNA Precursors/genetics RNA Splicing/genetics RNA-Binding Proteins/genetics Sequence Analysis, DNA Transcription Factors/genetics Transcription, Genetic/genetics Dewey 570 46 Département de biologie cellulaire 165 false Gene 211 1 fcc72134-780d-48e0-8f91-69f9a0a14e1b Article (Published version) Private fulltext.pdf 285284 application/pdf closedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene	Gene			1998	577	0

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Quentin, Mireille